Inhibitory control of actions is an executive process, which is important to attain behavioural goals. A defending basketball player, for example, who wants to block a jump shot, needs to inhibit the defensive action if the opponent only pretends to shoot. To investigate the ability of response inhibition in such a one-on-one situation in basketball, a computer-based anticipation experiment was conducted. In three blocks of 200 trails each, participants (N = 27, 11 females and 16 males, Mage = 22,26) viewed a video of a basketball jump shot (face-to-face, front view perspective) and were instructed to release the spacebar precisely at the point, where the ball leaves the fingertips of the basketball player (go-trials). In 25% of the trials, the video stopped prematurely before ball release, and participants were asked to withhold their response (stop-trials). The delay between the stop and the point of ball release was adjusted by a staircase tracking algorithm with a fixed step size, based on participants’ performance. For go-trials, results showed a constant error of 23 ms. For stop-trials, participants could only inhibit 50% of their responses when the video stopped between 183-200 ms before the point of ball release. Furthermore, a post-stop-trial adjustment (i.e., larger constant error) both, after successful and after unsuccessful stop-trials was found. Anticipation performance benefited from practice across the three blocks. Further experiments should investigate response inhibition in basketball using more realistic response actions.