Insects are essential to biodiversity and represent key elements of our food supply chains as pollination strongly contributes to global food production. On the flipside, insects are causing devastating swarms and pests affecting agricultural crop yields. Monitoring, understanding, and control of insect behavior is of burning importance for securing prosperous ecosystems and the development of control agents for sustainable pest management and food supply of a growing human population. Seven-transmembrane (7TM) receptors used to detect, communicate and execute timely behavior in diverse environments, as well as in relation to changing endogenous chemical cues are key to insect behavior. A better understanding of 7TM receptors' connections to insect behavior will improve our ability to model and arm insects with improved abilities to help tackle environmental concerns. Deorphanization of insect 7TM receptors using existing platforms (Drosophila “empty neuron”, frog oocyte, and HEK cells) is relatively low throughput. We are working toward developing a novel platform for insect 7TM receptor deorphanization based on the well-characterized and genetically tractable yeast Saccharomyces cerevisiae. In this study, we construct and characterize several calcium reporters in S. cerevisiae for reporting on the functionality of insect olfactory receptors (ORs), which are ligand-gated ion channels. We then expressed insect OR receptor co-receptors (ORCos) and several well-characterized olfactory receptors (ORs) and compare their functionality to that previously reported using more traditional characterization platforms. We also investigate the use of water/oil/water double emulsions for the maintenance of volatile odors during analysis.