The mouse accessory olfactory system plays a key role in detecting chemosignals during social interactions between conspecifics. Sensory information is detected by the system’s peripheral structure, the vomeronasal organ, and information is sent via the vomeronasal nerve to the accessory olfactory bulb (AOB). At this first central stage of information processing, AOB mitral cells (AMCs) receive excitatory synaptic input from vomeronasal sensory neurons via multiple glomeruli. Local interneurons surrounding glomeruli are collectively identified as periglomerular cells (PGCs). The physiological function(s) of this AOB neuron population remains elusive. Furthermore, it is unknown whether PGCs form a homo- or heterogeneous neural population. Here, we detail the biophysical properties of PGCs by performing whole-cell patch-clamp recordings from visually identified PGCs in acute slices of the mouse AOB. In addition, after labeling PGCs with biocytin via diffusion loading or single-cell electroporation, post-hoc morphological analysis allows correlation of structural and functional characteristics. Cell-type specific features are determined by analyzing passive and active membrane properties. Voltage-dependent currents, including potassium, sodium and calcium currents, display distinct activation and inactivation properties. With fast action potential kinetics, PGCs discharge at relatively high frequencies. Our results reveal both the biophysical properties and morphological features of an elusive AOB neuron population and, thus, provide first insight into physiological PGC characteristics.