19:10 - 21:00
Room: Ishikawa Ongakudō Interchange Hall
Poster Session
Investigating the roles of IFNγ and IFNγ-stimulated GTPases during Legionella pneumophila replication in alveolar macrophages and monocyte-derived cells
Chao Yang1, 2, Shivani Pasricha2, Sze Ying Ong2, Andrew Stephen Brown1, 2, Junya Yamagishi3, Chihiro Sugimoto3, Sammy Bedoui2, Ian R. van Driel1, Elizabeth L. Hartland2
1Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Vic, Melbourne, Australia, 2Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for infection and immunity, Vic, Melbourne, Australia, 3Global Institution for Collaborative Research and Education, Hokkaido University,, Hokkaido, Japan

Legionella pneumophila is an opportunistic human pathogen and the major cause of an acute pneumonia known as Legionnaire’s Disease. Upon inhalation, the bacteria replicate in alveolar macrophages (AM), within an intracellular vacuole termed the Legionella containing vacuoles (LCVs). Till now, little is known about how phagocytes control L. pneumophila lung infection. Recently, we found that monocyte-derived cells (MCs) are rapidly recruited into the lungs of L. pneumophila infected mice and that optimal clearance of the bacteria by MC, but not AMs and neutrophils, required interferon γ (IFNγ). To understand the mechanisms of MC restriction, the transcriptome of MCs obtained from L. pneumophila infected wildtype and ifnγ-/- mice was compared by RNA-sequencing. Gene ontology analysis showed enrichment of interferon-stimulated GTPases (ISGases) in MCs of wildtype mice compared to ifnγ-/- mice. Here, we found that Gbp1 and Irga6 overexpression contributes to the restriction of L. pneumophila replication in IFNγ-dependent manner in immortalized bone marrow derived macrophages (iBMDMs). While IFNγ is observed to disrupt effector protein translocation of Dot/Icm secretion system of L. pneumophila in iBMDMs, ISGases seem to have no effect on the effector protein translocation. Further, we found Gbp1 and Irga6 contribute to LCV rupture and autophagy to clean L. pneumophila by promoting the colocalization of Galectin-3 and Lamp1 with L. pneumophila in IFNγ activated iBMDMs. In addition, we found that IFNGR1 expression is significantly downregulated in AMs and neutrophils, but not MCs, during L. pneumophila lung infection. We confirmed downregulation of IFNGR1 in iBMDMs and showed that the downregulation relied on both MyD88 and Trif mediated NF-κB activation and was independent of type I IFN signalling, Irf3/7, Ripk3 and Bcl6 in macrophages. We propose that the reason AMs are unable to effectively restrict L. pneumophila replication in vivo is in part due to downregulation of IFNGR1.


Reference:
Tu-P6-2
Session:
Poster Session 6 “Cytokines in mucosal immunity”
Presenter/s:
Chao Yang
Presentation type:
Poster Presentation
Room:
Ishikawa Ongakudō Interchange Hall
Date:
Tuesday, 31 October 2017
Time:
19:10 - 21:00
Session times:
19:10 - 21:00