15:20 - 16:50
Room: ANA Crowne Plaza “Ohtori” Room A
Workshop Session
Chair/s:
Christopher Hunter, Reiko Shinkura
The role of BATF-3 dependent DC in the formation of fat associated lympoid clusters
Christopher Hunter, David Christian
University of Pennsylvania, Philadelphia, United States

The omentum is a visceral adipose tissue present in the peritoneal cavity that contains fat associated lymphoid clusters (FALCs). Despite the ability of the FALCs to form organized structures capable of T and B cell priming, little is understood about how inflammatory cells are recruited to these sites. Intraperitoneal immunization with a vaccine strain of Toxoplasma gondii results in a major reorganization of the FALCs that is dependent on Batf3-dependent cDC1s required for initial priming of a protective CD4 and CD8 Th1 type T cell response. The use Cre-expressing parasites shows that infected macrophages migrate from the peritoneum to the lymphoid clusters present in the omentum that results in rapid remodeling of these structures and T cell priming. Unexpectedly the cDC1s are a critical early source of IL-12 that induces IFN-g production required to induce the migration of antigen-laden macrophages from the peritoneum to the omentum. In Batf3-/- mice after the administration of recombinant IL-12p70 resulted in migration of peritoneal cells and recovery of CD4 T cell priming but was not sufficient for CD8 T cell priming. Together, these data identify a key role for cDC1 in co-ordinating the initial reorganization of FALCs required for their role in T cell priming.


Reference:
Tu-WS8-7
Session:
Workshop 8, “Cytokines and inflammatory factors in host defense”
Presenter/s:
Christopher Hunter
Presentation type:
Invited Speaker
Room:
ANA Crowne Plaza “Ohtori” Room A
Chair/s:
Christopher Hunter, Reiko Shinkura
Date:
Tuesday, 31 October 2017
Time:
16:30 - 16:50
Session times:
15:20 - 16:50