Dendritic cells (DCs) are heterogeneous cell populations, represented by different sub types each varying in terms of gene expression patterns and specific functions. DCs are classified into four major subtypes, the plasmacyotid dendritic cells (pDC), CD4+ DC, CD8α+ DC, CD4-CD8- double negative (DN) DC and tissue specific DCs. Transcription factors essential for the development of different DC subtype have been identified during the recent advancement of the field; yet molecular mechanism for the developmental program and dendritic cell functions remains poorly understood. Members of Interferon regulatory factors (IRF) play an essential role in generation of DC diversity and functions. Irf8 is essential for development of pDCs and CD8α+ DCs whereas Irf4 is critical to development of CD4+ DC subset. We recently demonstrated the hierarchical relationship between transcription factors important to development of CD8α+ DC development such that Irf8 is upstream of ATF-like 3 (Batf3) and Inhibitor of DNA binding 2 (Id2). Though, similar studies among transcription factors Irf4, Irf2 and Relb which are critical to development of CD4+ DC remains poorly understood. In current study, we analyzed the transcriptional regulation during CD4+ DC development and suggest cohesiveness between transcription factors Irf4, Irf2 and Relb in guiding DC subtype development.