19:10 - 21:00
Room: Ishikawa Ongakudō Interchange Hall
Poster Session
CD11b+Gr1dim Tolerogenic Dendritic Cell-like Cells are Expanded in Interstitial Lung Disease in SKG Mice
Sho Sendo, Jun Saegusa, Hirotaka Yamada, Yoshihide Ichise, Ikuko Naka, Takaichi Okano, Soshi Takahashi, Yo Ueda, Kengo Akashi, Akio Morinobu
Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe City, Japan

Objective. SKG mice develop interstitial lung disease (ILD) resembling rheumatoid arthritis-associated ILD (RA-ILD) in human. We identified a new cell population, CD11b+Gr1dim cells, in the lung of ILD-induced SKG mice. The aims of this study are to elucidate the roles of lung-infiltrating cells, especially CD11b+Gr1dim cells, in the pathogenesis of ILD in SKG mice.

Methods. We assessed the severity of zymosan A (ZyA)-induced ILD in SKG mice histologically, and examined lung-infiltrating cells by flow cytometry. Total lung cells and isolated monocytic myeloid-derived suppressor cells (M-MDSCs) were cultured in vitro with GM-CSF (and IL-4). The proliferation of CSFE-labeled naïve T cells co-cultured with isolated CD11b+Gr1dim cells and MDSCs was evaluated by flow cytometry. CD11b+Gr1dim cells were transferred to ZyA-treated SKG mice.

Results. MDSCs, Th17 cells, and group 1 and 3 innate lymphoid cells (ILC1s and ILC3s) were increased in the lungs; the proportion of these cells varied with ILD severity. In this process, we found that a unique cell population, CD11b+Gr1dim cells, were expanded in the severely inflamed lungs. About half of the CD11b+Gr1dim cells expressed CD11c. The CD11b+Gr1dim cells were induced from M -MDSCs with GM-CSF in vitro and were considered tolerogenic because they suppressed T-cell proliferation. The CD11b+Gr1dim cells have never described previously and termed CD11b+Gr1dim tolerogenic dendritic cell-like cells (CD11b+Gr1dim tolDC-LCs). Th17 cells, ILC1s and ILC3s in the inflamed lung produced GM-CSF, which may expand CD11b+Gr1dim tolDC-LCs in vivo. Furthermore, adoptive transfer of CD11b+Gr1dim tolDC-LCs significantly suppressed the progression of ILD in SKG mice.

Conclusion. We identified a unique cell population, termed CD11b+Gr1dim tolDC-LCs, in ILD of SKG mice, and the cells could be a potential target for the treatment of RA-ILD.

Poster Session 13 “Development and function of Macrophage and DC”
Sho Sendo
Presentation type:
Poster Presentation
Ishikawa Ongakudō Interchange Hall
Monday, 30 October 2017
19:10 - 21:00
Session times:
19:10 - 21:00