Dermanyssus gallinae(D.gallinae) is a small tick and is observed group, and at night, it is a vampire attached to the chicken. Recently, the poultry farm has been seriously infected with D.gallinae, which have reduced the productivity of chickens. And farmers are also using agricultural pesticides that are not approved as quasi-drugs for animals. So food safety, such as chicken meat and eggs, is also concerned.
Therefore, we want to develop the pesticide of D.gallinae using natural substances. we tested the pesticide effect on D.gallinae with 35 plant extracts. As a result, Gleditsia sinensis Lamark(G.sinensis) showed a high insecticidal rate of 89.6%. So we have started this study using G.sinensis to develop the pesticide of D.gallinae that are both safe and effective.
The G.sinensis was extracted with 95% EtOH and partitioned with n-hexane, CH2Cl2, EtOAc, n-BuOH and aqueous fractions. The purification of each fraction by column chromatography seperation and HPLC analysis to yield compound 1-6. These compounds are all the known compounds that were identified by analysis of NMR and HPLC data, along with comparison with those in the literatures.