Hyperuricemia is a chronic disease that seriously threatens human health.Vascular endothelial dysfunction is an important early stage in the development of chronic diseases such as hypertension, metabolic syndrome, and cardiovascular disease. Elevation of serum uric acid and impairment of vascular endothelial function occur at the same time. NOS uncoupling is the key link of uric acid damaging to endothelial function.Therefore, the countermeasures against the eNOS uncoupling and/or triggering factors may be an effective means to protect the vascular endothelial function and prevent and treat vascular diseasesin in patients with hyperuricemiain, which has become a research hotspot. Chinese medicine has always had obvious advantages in the treatment of chronic diseases. The Simiao Pills is a traditional Chinese medicine that treats hyperuricemia and gouty arthritis,and it can clear damp-heat. Rats were randomly divided into blank group, model group, benzbromarone group , and Si m iao Pill s group , a total of 4 groups. A hyperuricemia rat model was prepared with yeast extract 10g/(kg · d) combined with adenine 100mg/(kg · d).The rat serum and kidney tissues were collected for 14 consecutive days after successful modeling.The experimental results show that : Simiao Pills can reduce the serum levels of UA, ET-1, ROS, MDA in hyperuricemia model rats, increase serum NO, BH 4 levels, can effectively up-regulate the expression of eNOSmRNA and down-regulate the expression of Nox4mRNA in the aorta of hyperuricemia rats.Therefore, Si m iao Pills can reduce the serum uric acid in rats with hyperuricemia and protect the vascular endothelial function. The mechanism may be related to the NOS uncoupling.
Keywords: hyperuricemia ;NOS uncoupling; Endothelial function; Simiao pills
Acknowledgements: T he National Natural Science Foundation of China(No. 81273632 )