There are various factors that cause skin wrinkles such as UV irradiation, toxic substances, and genetic factors. Among them, UV irradiation is one of the most contributive elements causing wrinkles of the skin. It has been known that plants belonging to the Araliaceae family have various kinds of biological activities, therefore, they have long been used for many diseases. In this study, we investigated that Compound A isolated from the n-butanol fraction of Aralia elata has the protective role against the UVA-induced photo-oxidative stress in human dermal fibroblast cells. These cells are located in the dermis of the skin and have an important role in producing collagen, which contributes to maintaining the skin elasticity. However, the collagen can be degraded by the high concentration of the matrix metalloproteinase 1 (MMP1) in the cells caused by UV-induced reactive oxygen species. Therefore, it is important to find a substance that reduces MMP1 while promoting the collagen production in anti-wrinkle experiments. Our results revealed that the treatment with Compound A can increase the concentration of procollagen (precursor molecules of collagens) in both UV-irradiated and non-UV-irradiated cells compared to the control cells. Also the increase of MMP1 concentration via UV irradiation was significantly suppressed by Compound A. Furthermore, Compound A activated the nuclear factor-erythroid-2-related factor 2 (Nrf2), resulting in the expression of the antioxidant proteins. Taken together, these results indicate that Compound A is capable of protecting skin cells and preventing wrinkles induced by UV irradiation.