GA 2018

[Abstract] The chemical constituents of the water extraction of the aerial parts of Isodon henryi were investigated by various chromatographic methods including D-101 macroporous adsorptive resins, silica gel, Sephadex LH-20, and semi-preparative HPLC. As a result, ten compounds were separated and purified. By analyses of the UV, IR, MS, NMR spectra, their structures were determined as rabdosinate (1), lasiokaurin (2), enmenol-1-β-glucoside (3), epinodosinol (4), rabdosichuanin C (5), epinodosin (6), hebeirubescensins (k) (7), rubescensin C (8), enmenol (9), oridonin (10). Compounds 1-9 were isolated from Isodon henryi for the first time, as well as cytotoxicity of ten compounds. [Keywords] Isodon henryi; structural identification; diterpenes; cytotoxic References [1] Wang MT;Zhao TC;Li JC;et al. The Struetures of Rabdosinate and Rabdosinatol [J].Acta Chimica Sinica,1987(09):871-874. [2] Gao YH; Wei ZX; Cheng Y; et al. Cytotoxic ent-Kaurane Diterpenoids from Isodon nervosus[J]. Chem Biodivers, 2013, 10:1487-1492. [3] Liu HM, Yan X, Kiuchi F, et al. A new diterpene glycoside from rabdosia rubescens[J]. Chem Pharm Bull, 2000, 48(1), 148. [4] Fujita E, Fujita T, Taoka M, et al. Terpenoids XXIV, isolation of isodonal and epinodosin from Isodon japonicus and structure elucidation of sodoponin and epinodosinol, novel diterpenoids of the same plant[J]. Chem Pharm Bull, 1973, 21(6): 1357-1363. [5] Huang H, Zhang H, Sun H. Diterpenoids from rabdosia setschwanensis [J]. Phytochemistry, 1990, 29(8), 2591-2595. [6] Kubo I, Kamikawa T, Kubota T. Studies on constituents of Isodon japonicas Hara structures and absolute stereochemistry of isodonal, trichodonin and epinodosin. Tetrahedron 1974; 30(5):615-22. [7] Huang SX, Zhou Y, Pu JX, et al. Cytotoxic ent -kauranoid derivatives from isodon rubescens[J]. Tetrahedron, 2006, 62(20), 4941-4947. [8] Sun H, Chao J, Lin Z, et al. The structure of rubescensin c: a new minor diterpenoid isolated from rabdosia rubescens[J]. Chem Pharm Bull, 1982, 30(1), 341-343. [9] Wang XR, Wang HP, Hu HP, et al. Structures of macrocalyxin b, f, g and h, and maoyerabdosin from isodon macrocalyx[J]. Phytochemistry, 1995, 38(4), 921-926. [10] Fu JE; Fu JT; Kata YH; et al. Oridonin, a new diterpenoid from Isodon species [J]. Chemical Communications, 1967, (6): 252-254.