16:00 - 18:00
Poster Presentation-2
Room: Poster Area - Poster Shed
Poster Presentation
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ALKALOIDS FROM HYPECOUM PROCUMBENS L.
OZLEM DEMIRKIRAN 1, Ozlem GENCER 2
1 Trakya University, Faculty of Pharmacy, Department of Pharmacognosy, EDIRNE
2 Trakya University, Faculty of Science, Department of Chemistry, Edirne

In this study, the chemically investigation of Hypecoum procumbens L. was aimed. For this purpose, the plant material was soaked in EtOH for three weeks. Then EtOH extract acidificated and extracted with hexane after plant make basic and fractionated with dichloromethane, and ethyl acetate, respectively. Based on the results of TLC chromatography, it was observed that CH2Cl2 extract contain good amount of alkaloids in rich diversity. Several chromatographic methods have been used to isolate the alkaloids from this fraction.

Purification of dichloromethane extract of Hypecoum procumbens L. resulted in the isolation of four alkaloids including 8-methoxhy-11,12-methylenedieter (1), 8-acetonyl dihydro sanguinarine (2), oxohydrastin (3), deoxy protopine (4). The structure determination of the compounds achieved by spectral methods such as IR, UV, 1H-NMR, 13C-NMR, DEPT, HMBC, HSQC, COSY, ESI-MS.

The isolated compounds (1-4), were screened against AChE and BChEinhibitory activity. When tested against the AChE, compounds 1-4 displayed IC50 values of 1.13 μM, 1.80 μM, 0.92 μM, 0.88 µM, respectively. The IC50 values for BChEinhibition by compounds 1-4 were 6.81, 0.187, 4.9, 1.62 µM, respectively. Standard inhibitor (galanthamine) exhibited AChE and BChEinhibition with IC50 value of 3.5 μM and 36.0 μM, respectively.

References:

Zhang GL, Rücker G, Breitmaier E, Mayer R, Phytochemistry, 1995; 40, 1813-1816.

Nissanka APK, Karunaratne V, Bandara BMR, Kumar V, Nakanishi T, Nishi M, Inada A, Tillekeratne LMV, Wijesundara DSA, Gunatilika AAL, Phytochemistry, 2001; 56, 857-861.

Philipov S, Istatkova R, Denkova P, Dangaa S, Samdan J, Krosnova M, MunkH-Amgalan C, Nat Prod Res, 2009; 23, 982-987.

Reference:
Poster session-PO-36:
Session:
Poster Presentation-2
Presenter/s:
OZLEM DEMIRKIRAN
Presentation type:
Poster presentation
Room:
Poster Area - Poster Shed
Date:
Tuesday, 28th August, 2018
Time:
16:00 - 18:00
Session times:
16:00 - 18:00
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