Genus Tripleurospermum (Asteraceae) is represented in Turkey by 31 taxa, 15 of which are endemic [1]. In traditional Turkish folk medicine some species of the genus used for the treatment of cold and cough [2].
In the current study phenolic compounds of Tripleurospermum decipiens(Fisch. & Mey.) Bornm. (Asteraceae) were investigated by LC-MS/MS. Plant material was collected from Adana: Saimbeyli-Yeşilkent (2014) and voucher specimen was deposited in the herbarium of Faculty of Pharmacy, Istanbul University (ISTE 115054). Methanol extracts of the root and aerial parts of T. decipiens were investigated by LC-MS/MS. Experiments were performed with a Shimadzu 20A HPLC system coupled with an Applied Biosystems 3200 Q-Trap MS/MS detector. Separations were performed on a GL Science Intersil ODS 250 x 4,6 mm, i.d., 5 µm particle size column operating at 40º C at a flow rate of 0.7 mL/min. Detection was also carried out with a PDA detector. Elution was carried out using a binary gradient of the solvent mixture acetonitrile:water:formic acid (10:89:1, v/v/v) (solvent A) and acetonitrile:water:formic acid (89:10:1, v/v/v) (solvent B). The composition of B was increased from 10% to 100% in 40 min. Analyst 1.6 software was used for the data acquisition and analysis. DPPH and ABTS radical scavenging activity tests were performed for antioxidant activity.
According to the LC-MS/MS analysis, 5-Caffeoylquinic acid and 3,5-Dicaffeoylquinic acid were determined as the main components of both extracts. Luteolin, isorhamnetin and their glucosides were also identified in the aerial parts extract
. IC50 value of DPPH radical scavenging was determined 0.18 mg/ml for aerial part extract and 0.17 mg/ml for the root extract. ABTS radical scavenging activity was determined 0,51 mM Trolox Equivalent for aerial part extract and 0,41 mM Trolox Equivalent for root extract.
References:
[1] Kurkcuoglu M,Tosun, F, İnceer, H, Baser, K.H.C. Planta Med. 2016; 82: 718
[2] Altundag E, Ozturk, M. Procedia Soc. Behav. Sci. 2011; 197: 756-777