The genus Nepeta (Lamiaceae) is represented in Turkey by 33 species and altogether 38 taxa, 17 of these being endemic in Turkey [1,2]. It grows in Central and Southern Europe, West and Southern Asia. Nepeta species are widely used in folk medicine because of their antispasmodic, diuretic, antiseptic, antitussive, antiasthmatic activities [3].
Nepeta cadmea Boiss., endemic to Turkey was collected during the growing season from Denizli in 2017. The aerial parts of N. cadmea were hydrodistillated for 3 h using Clevenger-type apparatus to obtain essential oil. The essential oil was analyzed by GC, GC/MS, simultaneously to determined the chemical characterization of its. 4aα,7α,7aβ-nepetalactone (74.0%), 4aα,7α,7aα-nepetalactone (4.5%) and caryophyllene oxide (2.5%) were found as major components for essential oil. The potential in-vitro antibacterial activity of the essential oil was evaluated using the broth microdilution assay. A panel of human pathogenic strains Escherichia coli NRRLB-3008, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Bacillus cereus NRRL-B3711 and Streptococcus sanguinis ATCC 10556 were used. Minimal Inhibitory Concentrations (MIC) of the sample was determined, where ciprofloxacin was used as a positive control in the experiments. MIC values were found 2500, 1000, 600, 600, 600 μg/mL against E. coli, P. aeruginosa, S. typhimurium, B. cereus, S. sanguinis respectively. Compared the literature the essential oil had lower effective against these pathogens.
Key words : Nepeta cadmea, essential oil, antibacterial activity
References
[1] P. H. Davis. Flora of Turkey and East Aegean Islands. Edinburgh: University of Edinburgh Press; 1982:178.
[2] Güner A, Aslan S, Ekim T, Vural M, Babaç MT. Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul; 2012:564.
[3] Baser KHC, Kırımer N, Kürkçüoğlu M, Demirci B. Chemistry of Natural Compounds 2000; 36(4): 356-359.