Attenuation of Pseudomonas aeruginosa Virulence by Terminalia catappa leaves and Artocarpus alitilis flower Ethanolic Extracts
Abstract
One strategy to address the emergence of infectious diseases supported by the increasing cases of microbial antibiotic resistance is the development of anti-pathogenic drugs, a quorum quenching compound(s) capable of inhibiting microbial communication (quorum sensing inhibitor) (1,2). Previous anti-infective studies on Indonesian medicinal plants have focused mainly on anti-microbial drug discovery perspectives. However, no systemic effort has been made to explore the anti-quorum sensing activity of these plants. Our objective is to investigate several Indonesian medicinal plants’ ethanol extracts inhibitory activities against QS-mediated virulence factors in P. aeruginosa. Indonesian medicinal plant ethanolic extracts were tested for their capability to inhibit P. aeruginosa motility, biofilm formation using microtiter plate method, pyocyanin and LasA production using LasA staphylolytic assay. Statistical significance of the data were determined using one way ANOVA, followed by Dunnett’s test. Ethanolic extract of T. catappa leaves and A. alitilis flower capable to inhibit P. aeruginosa motility as well as pyocyanin production and biofilm formation. Both extracts also showed capability in reducing LasA protease production. T. catappa and A. alitilis are an interesting sources of innovative plant derived quorum quenching compound(s), thus can be used in the development of new antipathogenic drug.
Keywords: ethanol extract, anti-pathogenic drugs, quorum quenching, quorum sensing inhibitor, Pseudomonas aeruginosa
References
1. Waters CM, Bassler BL (2005). Quorum Sensing: Cell-to Cell Communication in Bacteria. Annu Rev Cell Dev Biol. 2005;21:319-46.
2.Adonizio, A.L., Downum, K., Benett, B.C., Mathee, K. (2006). Anti-quorum Sensing Activity of Medicinal Plants in Southern Florida, J Eth Pharmacol., 105: 427-435.