GA 2018

As fermented soybean (Glycine max), tempeh has been reported as a good source of isoflavones, which have shown anticancer activities in various cancer cells resulted from their activity as phytoestrogens. The exploration of cytotoxic effect of tempeh on T47D cells employing 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) resulted in IC50 values of 196.066 ± 15.956 µg/mL (1). In this research, we investigated the cytotoxic activity of isoflavones of tempeh such as daidzein and genistein and also their glucoside forms daidzin and daidzein on T47D breast cancer cell. In vitro experiments using MTT method showed that daidzein and genistein had potent cytotoxicity activity with IC50 values were 14.53 and 31.71 μM, respectively, whereas their glucoside forms had no cytotoxicity activity. (1) Yuliani S. H, Istyastono E. P, Riswanto F. D. O. The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein-Standardized Ethanolic Extract of tempeh -A Fermented Product of Soybean (Glycine Max). Orient J Chem 2016;32(3).