Açaí(Euterpe oleraceae Mart.) is a round and purple well-known palm fruit in Brazil and a commonly used herb in South America.Modern studies have shown that Açaí has various activities such as anti-oxidation,anti-inflammatory, anti-cancer and other activities.The Cyanidin-3-O-glucoside (C3G) is one of the anthocyanins were isolated from Açaí.This work aimed to propose a analytical methods for the quantitative and qualitative analysis of Cyanidin-3-O-glucoside (C3G) in extract of Açaí by HPLC,and to evaluate the different processing of extract of Açaí.In this study, Açai was degreased with petroleum ether firstly, then we added ten times 80% ethanol to extract in ultrasound for three times and 1h for each time.The condition for concentrating-drying processing is that respectively freeze-drying,drying under reduced pressure at 50°C and drying at 100°C. The HPLC conditions is that Dikma Diamonsil C18 column (150mm × 4.6 mm, 5 μm) was used as chromatographic column to separate the C3G. by gradient elution using 0.5% phosphoric acid solution as mobile phase A and water: acetonitrile(50:50,V/V) as mobile phase B at a flow rate of 0.8 mL/min.The column temperature was set at 30°C and the detection wavelength at 520 nm. The results showed that, C3G basically reached the baseline separation with a good linearity when the concentration was 5.375 -1 72 .000 u g/mL (r>0.9998) .The recovery rates were 99.84 % (RSD = 0.11 % ,n=6 ) . The C3G content and process transfer rate in extract of Açaí with drying under reduced pressure at 50°C processing and freeze-drying processing were respectively 0.94 mg/g,91.84% and 1.00mg/g ,97.86%.The C-3-G was not detected after drying at 100°C.These results indicated that ,the method is simple and reliable,and may be used for the quality control of the extract of Açaí , Which should be processed in low temperature environment. and Laying a foundation for further development and application of Açaí.
［ Key words ］ Açaí ; Cyanidin-3-O-glucoside; HPLC; determination;different processing methods