Anterior pituitary gland gonadotrope cells synthesize and secrete follicle stimulating hormone (FSH) and luteinizing hormone (LH), which are required for follicle development and subsequent ovulation in females. In mice, PAD expression is highest in gonadotrope cells during the estrus phase of the estrous cycle when ovulation occurs. In gonadotropes, histone citrullination regulates the expression of the LHb subunit gene and the riboprotein DiGeorge Syndrome Critical Region 8 (DGCR8) microprocessor complex subunit, which is required for canonical miRNA biogenesis. PADs also localize to the gonadotrope cytoplasmic compartment where they citrullinate the cytoskeletal proteins actin and tubulin, which may contribute to hormone vesicle trafficking and secretion. Our recent work shows that female Pad2/Pad4 double knockout (Pad2/4 DKO) mice are subfertile. Specifically, DKO females have delayed pubertal onset, disrupted estrous cycles, and smaller uteri as compared to controls. In pituitaries from female Pad2/4 DKO mice, gonadotrope specific gene expression is altered as compared to estrous cycle matched wild type controls. Changes in gonadotrope gene expression and irregular estrous cycles suggest that the loss of Pad2/4 in pituitary gonadotropes contributes to subfertility in female DKO mice.