Inter-individual differences in approaching reward cues as compared to rewards are referred to as sign- and goal-tracking behaviour. Goal-trackers approach the location of reward delivery, while sign trackers show a stronger focus on the location of the cue. While interest in these patterns has recently increased and tracking behaviour is usually categorized based on gaze behavior, no study has systematically investigated different variations of the paradigm. The main goal of the current study was to explore how the position where cue and reward were presented influence sign- and goal-tracking tendencies N = 38 participants completed a computerized Pavlovian Conditioned Approach task (with and without interactive components) in which the positions of the cue and reward (upper vs. lower screen location) were systematically varied. Based on gaze behavior towards the cue and reward positions, a gaze index was calculated to determine attentional biases towards different (cue vs. reward) locations. Analysis of the gaze behavior revealed a significant effect of position in the task without interactive component. Participants fixated the cue longer and faster if the cue was presented in the lower position of the screen compared to the upper position, while the reward location was fixated longer and faster if the CS was presented in the upper position. These findings challenge the assumption of the sign- and goal-tracking model that these tendencies represent stable, trait-like patterns, by demonstrating position-dependent shifts in gaze behavior.