Action control theories propose that responding to a stimulus leads to the binding of response and stimulus features into a common representation – a so-called event-file. Repeating any of its components leads to the retrieval of previous information, leading to so-called stimulus-response (S-R) binding effects. Previous research suggested that S-R binding is enhanced by positive compared to negative affect. However, other research found no such effect of affective modulations. We hypothesized that such a modulation might depend on the temporal aspect of presenting affective information. We asked participants to discriminate the shape of stimuli while orthogonally varying their color. In between stimuli we presented positive or negative images; in one block, the image was presented directly after prime offset, whereas in the other block, the image was presented immediately before probe onset. In a pre-registered, high-powered experiment (N = 134) we found no modulations of S-R binding effects by presenting positive or negative images. The results suggest that while valence might have an influence on overall performance, affective content does not affect the processes of binding and retrieval.