Molecular imaging continues to expand with growing numbers of imaging agents and strategies for the study of cancer in the living subject. A background will be provided on how one can take a molecular target, develop imaging agents against that target, and perform multimodality molecular imaging in both preclinical and clinical models. Applications to pre-clinical models of cancer as well as clinical translation will be discussed. Strategies for imaging with multiple modalities including radionuclide, optical, ultrasound, and photoacoustic imaging will be discussed. Methods to image cancer cells and immune cells including within the lymphatics will be highlighted. Through continued collaborations across multiple fields it should be possible to improve cancer detection and management.