09:00 - 10:45
Room: Amphi Mérieux
Chair/s:
Zayna CHAKER, Olivier RAINETEAU
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Submission 108
Role of PIEZO mechanosensitive channels in intestinal stem cells
S7-03-Oral Short Talk
Presented by: Meryem Baghdadi
Meryem Baghdadi 1, Ronja Houtekamer 2, Perrin Louisiane 1, Descroix Stephanie 3, Arshad Ayyaz 4, Alba Diz-Muñoz 5, Martijn Gloerich 2, Danijela Vignjevic 1, Tae-Hee Kim 6
1 Institut Curie, PSL Research University, CNRS UMR 144, Paris, France
2 Center for Molecular Medicine, University Medical Center Utrecht and Utrecht University, Utrecht, Netherlands.
3 Institut Curie, IPGG, PSL Research University, CNRS UMR 168, Paris, France.
4 Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
5 Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
6 Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Stem cells perceive and respond to biochemical and physical signals to maintain homeostasis. Yet, it remains

unclear how stem cells sense mechanical signals from their niche in vivo. In this work, we investigated the

roles of PIEZO mechanosensitive channels in the intestinal stem cell (ISC) niche. We used mouse genetics

and single-cell RNA sequencing analysis to assess the requirement for PIEZO channels in ISC maintenance.

In vivo measurement of basement membrane stiffness showed that ISCs reside in a more rigid

microenvironment at the bottom of the crypt. Three-dimensional and two-dimensional organoid systems

combined with bioengineered substrates and a stretching device revealed that PIEZO channels sense

extracellular mechanical stimuli to modulate ISC function. This study delineates the mechanistic cascade

of PIEZO activation that coordinates ISC fate decision and maintenance.