16:30 - 18:00
Talk Session 9
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16:30 - 18:00
Wed—HZ_2—Talks9—93
Wed-Talks9
Room:
Room: HZ_2
Chair/s:
Vaibhav A Diwadkar
Operant conditioning of planaria in the water-droplet paradigm
Wed—HZ_2—Talks9—9301
Presented by: Sarah Sophie Anton
Sarah Sophie Anton 1*Jannes Freiberg 1, 2
1 Christian-Albrechts-Universität zu Kiel, 2 CRC 1461
The process of learning, defined as a change in behavior due to experience, is a fundamental mechanism by which organisms adapt to their environment. Still little is known about the underlying neuronal mechanisms involved in learning. Therefore, less complex model organisms are crucial to deepen the understanding of learning processes. Numerous studies use planarians because of their remarkable regenerative abilities and their relatively simple nervous system. The prevailing view in the literature of the last 50 years suggests that planarians, such as Girardia tigrina, are capable of associative learning, including classical conditioning and operant conditioning. However, there have always been studies that have yielded inconsistent results or question the associative learning abilities of planarians. To consolidate the findings on associative learning in planarians, this study introduces a novel operant conditioning training-technique, the water-droplet paradigm. In the experiment, the aquatic planarian is placed on a dry surface, with water serving as reinforcement for movement on the dry surface. Additionally, a second training group received delayed reinforcement during the first trial to investigate the impeding effects of delayed reinforcement. To investigate if G. tigrina possess a form of long-term memory, a follow-up trial was conducted 24 hours after the initial training. The findings of this study indicate that G. tigrina are capable of associative learning when water is used as reinforcement, and that delayed reinforcement impairs a learning effect. However, there was no evidence of long-term memory in G. tigrina, possibly due to an insufficient number of training sessions.
Keywords: planarians, associative learning, operant conditioning, delayed reinforcement, long-term memory