In addition to the commonly used 2D photographs of suspects presented to eyewitnesses (Weinert, 2015), law enforcement agencies are increasingly employing various forms of 3D imagery. For instance, the Tokyo police uses 3D photographs of arrested individuals since 2016 (BBC, 2016). Thus, the application of 3D images in lineup procedures seems only a question of time. Previous studies have indicated that interactive lineups provide advantages over static 2D photo lineups (e.g., Colloff et al., 2021, 2022). Building on these findings, our study examined the effect of 3D versus 2D lineups on eyewitness accuracy within a between-subject design, employing a more ecologically valid virtual reality crime scenario. A sample of N = 116 participants first viewed a 360° video with two perpetrators committing a mock theft, followed by two sequential lineup sessions, two days later. Contrary to previous studies, our results revealed no significant differences in identification accuracy between 3D and 2D formats. This may be due to the more ecologically valid crime scenario presentation. However, we also hypothesize that the lack of interactive control over the 3D images could explain the absence of an effect. Unlike previous research, our participants were only exposed to 3D faces presented in a fixed time-loop video format. Our findings suggest that the advantage of 3D lineups over 2D may rely more on the ability to control viewing perspective than on the added depth information (cf., Zeng et al., 2020). This hypothesis will be explored in future research.