In literature, an experiment conducted by Dimonte, Adamatzky, Erokhin and Levin (2016) shows evidence of lateralized movement of the unicellular slime-mold Physarum polycephalum in T-labyrinths. While behavioral lateralization is a common phenomenon observed in higher animals, it is far from ubiquitous, and not yet reported in non-animals. Notwithstanding the authors' assertion that this phenomenon would be a natural consequence of the cytoskeleton of single-celled organisms, no other observations on this subject have been reported in the literature. Moreover, it would be astonishing to discover behavioral lateralization in a formless organism, predating even animals. To further investigate this, an in-depth replication experiment was conducted. In consideration of the amoeboid organism's diverse sensory capabilities, we controlled for the influence of light, vibration, artificial magnetic fields and the magnetic field of the earth. To induce genetic diversity, slime molds originating from France and Germany were used. The experiments conducted on over 1,000 individual organisms demonstrate the absence of generalized behavioral lateralization in both genotypes. However, due to our results we suggest an effect of illumination, vibration and magnetic fields on movement. Therefore, we come to the conclusion that slime molds show no inherent lateralized movement, and that lateralization is an artifact of the experimental conditions.