Multifunctional Effects of Lactococcus Ferment Lysate for Skin Whitening
58
Presented by: Fan Yang
Abstract:
The inflammation produced by exposure to ultraviolet (UV) light has been well documented clinically and histologically. These acute or chronic inflammatory responses cause inflammatory cytokine production from epidermal keratinocytes as well as dermal fibroblasts and other cells, which in turn stimulate melanocytes, often resulting in skin pigmentation. However, not all inflammatory cytokines increase skin pigmentation. Interleukin (IL)-1, IL-6, and tumor necrosis factora (TNFa) are known to suppress skin pigmentation, which suggests the importance of further elucidating the relationship between melanogenesis and inflammation. In this study, we screened the cosmetic substances which activate TNFa and evaluated the effect on human skin. In the result, we found Lactococcus Ferment Lysate can inhibit the DNA damage and cell apoptosis caused by UV, activate TNFa and enhance cellular immune function, reduce skin pigmentation after the inflammation. In addition,3D epidermal skin model was irradiated with UVB(50mJ/cm2) for 3 days, the melanin model was photographed, L* was measured with chromatic meter, content of melanin was measured, blackness distribution was detected after section staining. We found the Lactococcus Ferment Lysate can reduce the content and distribution of melanin, lighten skin.
Introduction:
The color of skin is determined by genes(which determine the physiology, structure and functional difference of the skin), external factor(such as UV) and skin inflammation. The object of our study is to find the multi-dimensional effect for skin whitening based on a lysate of Lactococcus lactis. The results show that Lactococcus Ferment Lysate have various whitening activities.
Methods:
Evaluate the inhibiting effect of DNA damage: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of DNA replication-dependent nucleosome assembly and organization, DNA packaging complex and nucleosome were measured using RNA-seq.
Evaluate the inhibiting effect of cell apoptosis: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress, cellular response to glucose starvation and PERK-mediated unfolded protein response were measured using RNA-seq.
Evaluate the inhibiting and activation effect of inflammation: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of chemotaxis and migration of neutrophils, signal path of IL-17 and NF-κB and TNFa were measured using RNA-seq.
Evaluate the acceleration effect of immune: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of leukocyte chemotaxis and migration, CXCR chemokine receptors binding, chemokine receptors binding , GPCRs binding and cytokine activity were measured using RNA-seq.
Evaluate the acceleration effect of skin whitening after UVB: 3D epidermal skin model was irradiated with UVB(50mJ/cm2) for 3 days, the melanin model was photographed, L* was measured with chromatic meter, content of melanin was measured, blackness distribution was detected after section staining.
Results:
Lactococcus Ferment Lysate is shown to inhibit the DNA damage of keratinocytes after UVB, by increasing the related genes of DNA replication-dependent nucleosome assembly and organization, DNA packaging complex and nucleosome significantly, inhibit cell apoptosis by decreasing the related genes of intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress, cellular response to glucose starvation and PERK-mediated unfolded protein response. In addition, it can activation TNFa ，Inhibit inflammation of fibroblast, separately by increasing the related genes of chemotaxis and migration of neutrophils, signal path of IL-17 and decreasing signal path of NF-κB, strengthen skin immune function by increasing related genes of leukocyte chemotaxis and migration, CXCR chemokine receptors binding, GPCRs binding, chemokine receptors binding and cytokine activity.
Discussion and Conclusion:
These results demonstrate that Lactococcus Ferment Lysate could be an effective ingredient for cosmetics that exerts a wide variety of biological activities used for skin whitening. It can inhibit the DNA damage and cell apoptosis caused by UV, fight against inflammation and enhance cellular immune function, reduce skin pigmentation after the inflammation, reduce the content and distribution of melanin, lighten skin.
GPCRs :G Protein-Coupled Receptors,
The inflammation produced by exposure to ultraviolet (UV) light has been well documented clinically and histologically. These acute or chronic inflammatory responses cause inflammatory cytokine production from epidermal keratinocytes as well as dermal fibroblasts and other cells, which in turn stimulate melanocytes, often resulting in skin pigmentation. However, not all inflammatory cytokines increase skin pigmentation. Interleukin (IL)-1, IL-6, and tumor necrosis factora (TNFa) are known to suppress skin pigmentation, which suggests the importance of further elucidating the relationship between melanogenesis and inflammation. In this study, we screened the cosmetic substances which activate TNFa and evaluated the effect on human skin. In the result, we found Lactococcus Ferment Lysate can inhibit the DNA damage and cell apoptosis caused by UV, activate TNFa and enhance cellular immune function, reduce skin pigmentation after the inflammation. In addition,3D epidermal skin model was irradiated with UVB(50mJ/cm2) for 3 days, the melanin model was photographed, L* was measured with chromatic meter, content of melanin was measured, blackness distribution was detected after section staining. We found the Lactococcus Ferment Lysate can reduce the content and distribution of melanin, lighten skin.
Introduction:
The color of skin is determined by genes(which determine the physiology, structure and functional difference of the skin), external factor(such as UV) and skin inflammation. The object of our study is to find the multi-dimensional effect for skin whitening based on a lysate of Lactococcus lactis. The results show that Lactococcus Ferment Lysate have various whitening activities.
Methods:
Evaluate the inhibiting effect of DNA damage: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of DNA replication-dependent nucleosome assembly and organization, DNA packaging complex and nucleosome were measured using RNA-seq.
Evaluate the inhibiting effect of cell apoptosis: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress, cellular response to glucose starvation and PERK-mediated unfolded protein response were measured using RNA-seq.
Evaluate the inhibiting and activation effect of inflammation: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of chemotaxis and migration of neutrophils, signal path of IL-17 and NF-κB and TNFa were measured using RNA-seq.
Evaluate the acceleration effect of immune: Human keratinocytes were irradiated with UVB(300mJ/cm2), related genes of leukocyte chemotaxis and migration, CXCR chemokine receptors binding, chemokine receptors binding , GPCRs binding and cytokine activity were measured using RNA-seq.
Evaluate the acceleration effect of skin whitening after UVB: 3D epidermal skin model was irradiated with UVB(50mJ/cm2) for 3 days, the melanin model was photographed, L* was measured with chromatic meter, content of melanin was measured, blackness distribution was detected after section staining.
Results:
Lactococcus Ferment Lysate is shown to inhibit the DNA damage of keratinocytes after UVB, by increasing the related genes of DNA replication-dependent nucleosome assembly and organization, DNA packaging complex and nucleosome significantly, inhibit cell apoptosis by decreasing the related genes of intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress, cellular response to glucose starvation and PERK-mediated unfolded protein response. In addition, it can activation TNFa ，Inhibit inflammation of fibroblast, separately by increasing the related genes of chemotaxis and migration of neutrophils, signal path of IL-17 and decreasing signal path of NF-κB, strengthen skin immune function by increasing related genes of leukocyte chemotaxis and migration, CXCR chemokine receptors binding, GPCRs binding, chemokine receptors binding and cytokine activity.
Discussion and Conclusion:
These results demonstrate that Lactococcus Ferment Lysate could be an effective ingredient for cosmetics that exerts a wide variety of biological activities used for skin whitening. It can inhibit the DNA damage and cell apoptosis caused by UV, fight against inflammation and enhance cellular immune function, reduce skin pigmentation after the inflammation, reduce the content and distribution of melanin, lighten skin.
GPCRs :G Protein-Coupled Receptors,