Collagen and its derivative proteins have been widely used as a major compound for skin care products as a natural ingredient and moisturizer. Most commercially available collagens are animal-derived collagen type I and other forms of collagen, such as type III collagen, are far less prevalent in animals, making extraction and purification extremely difficult and expensive. We report the production of a 50kDa protein produced in yeast that is100% identical to the N-terminus of the human type III collagen. This recombinant protein has a larger molecular weight than most incumbent recombinant collagen proteins available for personal care applications.We have manged the industrialization of both the fermentation and purification processes to produce a final recombinant protein product.This final protein product was shown to be safe for application to human skin and compatible with common formulations. This recombinant collagen type III protein was also shown to uniquely stimulate both collagen type I and type III production and secretion by primary human dermal fibroblasts. The unique combination of biostimulation, compatibility with beauty product formulations and demonstrated commercial production, make this novel recombinant type III collagen a good candidate for broad application in dermatology and in cosmetics.