A New Strategy to Promote Collagen Production and Wound Healing by the Combined Treatment of Ascorbic Acid and Glycinamide in Human Dermal Fibroblasts
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Presented by: Yong Chool Boo
Background: A decrease of dermal collagen is a common feature of natural aging and photoaging of the skin.
Objectives: To present a novel strategy to enhance dermal collagen production.
Methods: To identify amino acid analogs with excellent collagen production enhancing effects, human dermal fibroblasts (HDFs) were treated with 20 kinds of amidated amino acids individually at 1 mM. Cell viability, collagen production, and mRNA expression of procollagen genes (COL1A1, COL3A1) were compared when glycinamide, glycine analogs, ascorbic acid (AA), AA analogs, and transforming growth factor (TGF)-β1 were treated each or in combinations.
Results: Of the 20 different amidated amino acids, glycinamide enhanced collagen production most effectively. Glycine, a free amino acid, enhanced collagen production to a lesser degree but other glycine analogs, such as N-acetyl glycine, N-acetyl glycinamide, glycine methyl ester, glycine ethyl ester, and glycyl glycine, did not show such effect. AA and TGF-β1 increased COL1A1 and COL3A1 mRNA expression and collagen production, whereas glycinamide increased collagen production without changes in the mRNA expression. The combination of AA and glycinamide synergistically enhanced collagen production, cell proliferation, and wound healing in HDFs to a similar level in cells treated with TGF-β1. AA analogs, such as magnesium ascorbyl phosphate, 3-O-ethyl ascorbic acid, ascorbyl glucoside, and ascorbyl tetra-isopalmitate, enhanced collagen production, especially when they were co-treated with glycinamide.
Conclusions: This study provided a new strategy to enhance cell collagen production using glycinamide in combination with AA or its analogs. This strategy may be useful in antiaging cosmetics.
Objectives: To present a novel strategy to enhance dermal collagen production.
Methods: To identify amino acid analogs with excellent collagen production enhancing effects, human dermal fibroblasts (HDFs) were treated with 20 kinds of amidated amino acids individually at 1 mM. Cell viability, collagen production, and mRNA expression of procollagen genes (COL1A1, COL3A1) were compared when glycinamide, glycine analogs, ascorbic acid (AA), AA analogs, and transforming growth factor (TGF)-β1 were treated each or in combinations.
Results: Of the 20 different amidated amino acids, glycinamide enhanced collagen production most effectively. Glycine, a free amino acid, enhanced collagen production to a lesser degree but other glycine analogs, such as N-acetyl glycine, N-acetyl glycinamide, glycine methyl ester, glycine ethyl ester, and glycyl glycine, did not show such effect. AA and TGF-β1 increased COL1A1 and COL3A1 mRNA expression and collagen production, whereas glycinamide increased collagen production without changes in the mRNA expression. The combination of AA and glycinamide synergistically enhanced collagen production, cell proliferation, and wound healing in HDFs to a similar level in cells treated with TGF-β1. AA analogs, such as magnesium ascorbyl phosphate, 3-O-ethyl ascorbic acid, ascorbyl glucoside, and ascorbyl tetra-isopalmitate, enhanced collagen production, especially when they were co-treated with glycinamide.
Conclusions: This study provided a new strategy to enhance cell collagen production using glycinamide in combination with AA or its analogs. This strategy may be useful in antiaging cosmetics.