Novel glycerin compounds improve skin health
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Presented by: gotaro shiota
Background: Novel glycerin compounds (NGCs) were developed which generated new reaction products, endoperoxide derivatives. NGCs have been reported to exhibit various biological effects. In addition to their deodorizing, disinfecting and antiviral effects, NGCs were found to increase type I collagen production and inhibit pro-inflammatory cytokine secretion.
Aims: This study is aimed to evaluate the effects of NGCs on skin homeostasis by investigating antioxidant activity and barrier functions
Methods: In this study, in order to clarify the effects of NGCs on normal human skin, we quantified the mRNA gene expression of heme oxygenase-1 (HO-1) and DAD(P)H quinone dehydrogenase 1 (NQO-1) as antioxidant factors by PR-PCR and the protein content of intracellular glutathione (GSH) using a culture system of normal human epidermal keratinocyte. Moreover, gene expression of factors related to skin barrier functions (involucrin (INV), filaggrin (FLG), and serine palmitoyl transferase (SPT)) was examined to clarify the effects of NGCs on epidermal cell differentiation.
Results and Discussion: The NGCs enhanced expression of HO-1, NQO-1 mRNA and GSH protein in a concentration-dependent manner from 20 ppm after 24 h of stimulation. These results imply that the oxidative property of NGCs activates Nrf2 (NF-E2-related factor 2) transcription factor, which is a biological defense sensor, and induces the expression of antioxidant factors. Since their expression was caused by oxidative stimulation at low concentrations of NGCs, it was strongly suggested that their hormesis effect would be working protectively for the skin. Consistent with this, the expression of INV, FLG, and SPT genes also increased at low concentrations of NGCs: FLG showed a threefold increase compared to the control group. These results indicate that NGCs promote epidermal cell differentiation and have positive effects on skin barrier functions. We are currently conducting detailed analysis of the effects of NGCs using three-dimensional cultures of human epidermis.
Conclusion: Mild oxidative stimulation of NGCs could lead to healthy skin through their hormesis effect on antioxidant activity and barrier functions.
Aims: This study is aimed to evaluate the effects of NGCs on skin homeostasis by investigating antioxidant activity and barrier functions
Methods: In this study, in order to clarify the effects of NGCs on normal human skin, we quantified the mRNA gene expression of heme oxygenase-1 (HO-1) and DAD(P)H quinone dehydrogenase 1 (NQO-1) as antioxidant factors by PR-PCR and the protein content of intracellular glutathione (GSH) using a culture system of normal human epidermal keratinocyte. Moreover, gene expression of factors related to skin barrier functions (involucrin (INV), filaggrin (FLG), and serine palmitoyl transferase (SPT)) was examined to clarify the effects of NGCs on epidermal cell differentiation.
Results and Discussion: The NGCs enhanced expression of HO-1, NQO-1 mRNA and GSH protein in a concentration-dependent manner from 20 ppm after 24 h of stimulation. These results imply that the oxidative property of NGCs activates Nrf2 (NF-E2-related factor 2) transcription factor, which is a biological defense sensor, and induces the expression of antioxidant factors. Since their expression was caused by oxidative stimulation at low concentrations of NGCs, it was strongly suggested that their hormesis effect would be working protectively for the skin. Consistent with this, the expression of INV, FLG, and SPT genes also increased at low concentrations of NGCs: FLG showed a threefold increase compared to the control group. These results indicate that NGCs promote epidermal cell differentiation and have positive effects on skin barrier functions. We are currently conducting detailed analysis of the effects of NGCs using three-dimensional cultures of human epidermis.
Conclusion: Mild oxidative stimulation of NGCs could lead to healthy skin through their hormesis effect on antioxidant activity and barrier functions.