Study of Natural Antibacterial Body Wash Against Pathogenic Bacteria, Fungi and Covid-19 Virus (SAR-CoV-2) and Its Efficacy to Protect Skin from Pathogenic Bacterial
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Presented by: Wiwin Rosmayanti
Introduction: The onset of COVID-19 worldwide pandemic has increased awareness of personal hygiene, not only related to Covid-19 virus but also to other pathogenic bacteria that may cause diseases. Various hygiene personal care products such as sanitizer, hand wash and body wash have emerged and are widely used. Many studies show that body wash products are effective in killing bacteria and fungi but the effect on viruses is still limited. This paper presents a study on the analysis of an antibacterial body wash on killing bacteria, fungi and Covid-19 virus as well as skin protection from pathogenic bacteria. The body wash formula contains soap and surfactants also Mentha piperita (leaf) extract as natural active antibacterial.
Method: The antibacterial activity of Mentha piperita (leaf) extract to some Gram-positive and Gram-negative bacterial strain was evaluated by using dilution method to detect the Minimum Inhibitory Concentration (MIC). The extract was combined with soap and surfactants to formulate body wash. The body wash was then evaluated by a quantitative suspension test method to determine bactericidal, fungicidal and virucidal activity refers to EN 1276:2019, EN 1650:2008 and EN 14476:2013+A2:2019 respectively. Furthermore, this study evaluates the body wash effect on protection of skin from pathogenic bacteria. A total of 20 panelists used it on the skin followed by collection of skin swabs after 12 hours. The swabs were grown in selective agar media to determine the growth of pathogenic bacteria.
Results: The MIC of Mentha piperita (leaf) extract against S. aureus, P. aeruginosa, and E. coli was 3mg/ml, 100ml/ml and 25mg/ml respectively, showing that the extract has the ability to inhibit the bacteria. By quantitative suspension test, the body wash was found to be effective in killing >99.999% of E. coli, S. aureus, P aeruginosa, S. typhimurium and C. acnes also >99.991% of C. albicans and A. brasiliensis, within 60 second contact time. It was also effective in inactivating Covid-19 virus by reducing 4.09 log10 within 60 second contact time. Skin protection efficacy tested on panelists shows that after 12 hours of applications there is no pathogenic bacteria detected.
Conclusion: This study indicates that the combination of soap, surfactant and Mentha piperita (leaf) extract in a body wash is effective in protecting skin from pathogenic bacteria, fungi and Covid-19 virus.
Method: The antibacterial activity of Mentha piperita (leaf) extract to some Gram-positive and Gram-negative bacterial strain was evaluated by using dilution method to detect the Minimum Inhibitory Concentration (MIC). The extract was combined with soap and surfactants to formulate body wash. The body wash was then evaluated by a quantitative suspension test method to determine bactericidal, fungicidal and virucidal activity refers to EN 1276:2019, EN 1650:2008 and EN 14476:2013+A2:2019 respectively. Furthermore, this study evaluates the body wash effect on protection of skin from pathogenic bacteria. A total of 20 panelists used it on the skin followed by collection of skin swabs after 12 hours. The swabs were grown in selective agar media to determine the growth of pathogenic bacteria.
Results: The MIC of Mentha piperita (leaf) extract against S. aureus, P. aeruginosa, and E. coli was 3mg/ml, 100ml/ml and 25mg/ml respectively, showing that the extract has the ability to inhibit the bacteria. By quantitative suspension test, the body wash was found to be effective in killing >99.999% of E. coli, S. aureus, P aeruginosa, S. typhimurium and C. acnes also >99.991% of C. albicans and A. brasiliensis, within 60 second contact time. It was also effective in inactivating Covid-19 virus by reducing 4.09 log10 within 60 second contact time. Skin protection efficacy tested on panelists shows that after 12 hours of applications there is no pathogenic bacteria detected.
Conclusion: This study indicates that the combination of soap, surfactant and Mentha piperita (leaf) extract in a body wash is effective in protecting skin from pathogenic bacteria, fungi and Covid-19 virus.