Macromolecular collagen, a functional protein with molar weight around 300000, is the main composite of consumption medical (dressings, injections, water acupuncture) and plays a significant role in traditional medical health market. In this study, a series of L-carnitin-matrix ionic liquids, as supramolecular solvents, were developed based on the density functional theory (DFT) calculation results. This system is designed to solve the drawbacks of macromolecular collagen, such as low bioavailability, difficulty in permeating epidermis and being absorbed by dermis, and poor stability during transportation. The L-carnitin-based supramolecular solvent loading macromolecule collagen has a particle size of less than 70nm accompanying good dispersity and biocompatibility. When the concentration of the collagen in the IL is 11.91mg/ mL, the encapsulation rate is 69.5%, and the inactivation rate is 23.2% after 6 hours at 45℃. The solubility and the skin permeability of macromolecule collagen are increased by 3.4 times. The content of collagen in cuticle, epidermis and dermis are increased by 1.2 times, 2.7 times and 4.3 times, respectively, and the bioavailability is increased by nearly 5 times. This system can also effectively relieve wrinkles and pigmentation caused by aging. This study also explores the mechanism of IL solubilization in promoting infiltration. With hydrophilic and lipophilic groups, the resulting IL carries out non-biding lipids to the collagen to enhance the encapsulation rate. In addition, it opens the tight junction to promote collagen bypass, and improves the permeability of keratinocytes when promoting across cell transport of collagen. In conclusion, this study breaks the combined limitations of biological ILs, provides a new solution for solving, stabilizing and permeating generic proteins and macromolecular drugs in dressing, manual organ regeneration medicine, tissue engineering, biological skin care, etc.