Objective: 16SrDNA sequencing technology was used to analyze the characteristics of sensitive patients'n flora with allergic skin, and providing basic data for cosmetics research and application guidance. Methods: Forty female volunteers aged between 25 and 50 were recruited, which were divided ito the sensitive group (19) and the health group (20). Swabs were used to collect skin samples of the volunteers' cheeks, and 16S rDNA sequencing technology was used to detect the skin flora and software was used to analyze the data. Results: ① A total of 86,525 valid sequences were detected by high out-put sequencing technology, and 2005 OTUs were obtained by sequence clustering. A total of 1190 OTUs were annotated to the genus level. ② The analysis of skin flora α diversity showed that there was no significant difference in the richness and diversity between the sensitive group and the healthy group. ③ There were significant differences between the sensitive group and the healthy group by skin flora β diversity analysis.④ In the analysis of the different bacteria at the phylum level, the number of phylum and spore increased significantly in the sensitive group. ⑤ Analysis of the different bacteria at the genus level showed that the rumen bacteria and Lactobacillus were significantly increased in the sensitive group, while the streptococcus granulocytes and Roxella were significantly decreased. ⑥ Analysis of bacterial flora proportion showed that the ratio of propionibacterium to staphylococcus in the healthy group was R=1-4, while the ratio of propionibacterium to staphylococcus in the sensitive group was R < 1 or R > 4, indicating that the ratio of propionibacterium to staphylococcus was unbalanced, suggesting that R value might be related to skin sensitivity. Conclusions The facial flora of sensitive patients was disordered, which was manifested as changes in the community structure of the flora: increase in the Tenericutes and Clostridium, and imbalance in the proportion of propionibacterium and staphylococcus, which may be related to the occurrence and development of skin sensitivity.