Investigation on anti-inflammatory and whitening effects of active compounds from Artemisia argyi
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Presented by: yue li
Investigation on anti-inflammatory and whitening effects of active compounds from Artemisia argyi
Li Yue*,1 Yan xin1 FengZhe1 Yang Xin1 Wu Qiang1
1 PROYA International Academy of Science, PROYA Cosmetics Co., Ltd, Hangzhou, China.
Abstract：
Introduction: Artemisia argyi is a troditional Chinese herbal as a commonly used medicinal and edible herbal, it has been used for thousands of years in treating irregular menstruation, dyamenorrhea and nontraumatic hemorrhage. The coumarins, flavonoids, triterpenoids, sesquiterpenoids isolated from Artemisia argyi has been researched previously, some of them showed an extremely effect on anti-inflammatory, antitumour, antimutagen and antimicrobial. Previous studies by my colleagues have shown that artemisia argyi extract can inhibit tyrosinase activity，but its mechanism of action and other potential cosmetic activity has not been thoroughly studied. In this study, we tried to investigate on anti-inflammatory and whitening effects and its possible mechanisms of active compounds from Artemisia argyi
Methods: The dried powder of Artemisia argyi was extracted three times with 85% aqueous ethanol at 45℃ assisted by Ultrasound. The extracts were concentrated until no more ethanol was left. The residual solid was dissolved in water and partitioned sequentially with petroleum ether, ethyl acetate and n-butanol. The anti-melanogenic and melanin inhibitory effect in B16F10 melanocytes. Anti-inflammatory abilities were determined by NO inhibitory effect in mouse macrophages RAW264.7 cells.
Then few compounds were selected from ethyl acetate extract. Through the test we found several compounds which have anti-melanogenic and anti-inflammatory abilities. After that we evaluated the melanogenesis-related genes, such as Tyr, Trp-1, Trp-2 and Inflammatory-related genes such as interleukins using real-time qPCR.
Results: The ethyl acetate extract from Artemisia argyi was conformed to have an excellent anti-melanogenic effect and also found that it has very good NO inhibitory effect. As a result, the compounds in ethyl acetate extract from Artemisia argyi, such as Nepetin was confirmed to have melanin inhibitory effect, such as Jaceosidin and Quercetin were shown to have NO inhibitory effect. These compounds from Artemisia argyi were further analyzed for gene expression. The results showed that Nepetin could down-regulate the expression of tyrosinase-related genes and that Jaceosidin and Quercetin could inhibited the expression levels of interleukins.
Discussion and Conclusion: These results illustrated that Artemisia argyi compounds have anti-melanogenic and anti-inflammatory abilities. By down regulating MITF related genes, the compounds in Artemisia argyi that we choose were conformed to have the ability of melanogenesis inhibit. But of course, not the strongest one. The compounds Jaceosidin and Quercetin were conformed to have the anti-inflammatory ability by down regulating interleukins. The results provide some mechanism basis for the application of compounds from Artemisia argyi in cosmetics.
Keywords: (Artemisia argyi; Anti-inflammatory; Anti-melanogenic; Nepetin).
*corresponding author E-mail:18597100636@163.com
Li Yue*,1 Yan xin1 FengZhe1 Yang Xin1 Wu Qiang1
1 PROYA International Academy of Science, PROYA Cosmetics Co., Ltd, Hangzhou, China.
Abstract：
Introduction: Artemisia argyi is a troditional Chinese herbal as a commonly used medicinal and edible herbal, it has been used for thousands of years in treating irregular menstruation, dyamenorrhea and nontraumatic hemorrhage. The coumarins, flavonoids, triterpenoids, sesquiterpenoids isolated from Artemisia argyi has been researched previously, some of them showed an extremely effect on anti-inflammatory, antitumour, antimutagen and antimicrobial. Previous studies by my colleagues have shown that artemisia argyi extract can inhibit tyrosinase activity，but its mechanism of action and other potential cosmetic activity has not been thoroughly studied. In this study, we tried to investigate on anti-inflammatory and whitening effects and its possible mechanisms of active compounds from Artemisia argyi
Methods: The dried powder of Artemisia argyi was extracted three times with 85% aqueous ethanol at 45℃ assisted by Ultrasound. The extracts were concentrated until no more ethanol was left. The residual solid was dissolved in water and partitioned sequentially with petroleum ether, ethyl acetate and n-butanol. The anti-melanogenic and melanin inhibitory effect in B16F10 melanocytes. Anti-inflammatory abilities were determined by NO inhibitory effect in mouse macrophages RAW264.7 cells.
Then few compounds were selected from ethyl acetate extract. Through the test we found several compounds which have anti-melanogenic and anti-inflammatory abilities. After that we evaluated the melanogenesis-related genes, such as Tyr, Trp-1, Trp-2 and Inflammatory-related genes such as interleukins using real-time qPCR.
Results: The ethyl acetate extract from Artemisia argyi was conformed to have an excellent anti-melanogenic effect and also found that it has very good NO inhibitory effect. As a result, the compounds in ethyl acetate extract from Artemisia argyi, such as Nepetin was confirmed to have melanin inhibitory effect, such as Jaceosidin and Quercetin were shown to have NO inhibitory effect. These compounds from Artemisia argyi were further analyzed for gene expression. The results showed that Nepetin could down-regulate the expression of tyrosinase-related genes and that Jaceosidin and Quercetin could inhibited the expression levels of interleukins.
Discussion and Conclusion: These results illustrated that Artemisia argyi compounds have anti-melanogenic and anti-inflammatory abilities. By down regulating MITF related genes, the compounds in Artemisia argyi that we choose were conformed to have the ability of melanogenesis inhibit. But of course, not the strongest one. The compounds Jaceosidin and Quercetin were conformed to have the anti-inflammatory ability by down regulating interleukins. The results provide some mechanism basis for the application of compounds from Artemisia argyi in cosmetics.
Keywords: (Artemisia argyi; Anti-inflammatory; Anti-melanogenic; Nepetin).
*corresponding author E-mail:18597100636@163.com