In vitro effect of antioxidant activities on Chinese herbal medicine extracts
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Presented by: Miin Chiu
Introduction
Chinese herbal medicine (CHMs) is widely used in Taiwan, and they are closely related to our life. A variety of Chinese herbal medicine has different effects, such as: Richardia scabra (Rs) for traditional folk gynecological medicine, has the effect of anti-inflammatory; Taxillus chinensis (Tc) can nourish the liver and kidneys, dredge the meridians, strengthen the muscles and bones; Arundo donax (Ad) and Anemarrhena asphodeloides (Aa) are commonly used to purge internal heat. In this study, we choose above mentioned four Chinese herbal medicines to carry out the biological evaluations of water and ethanol extracts of 4 CHMs by simple and widely popular method for screening free radical-scavenging ability and ferric reducing antioxidant power (FRAP). The objective of this study is to develop the new raw materials that can be used in cosmetic industry.
Methods
The antioxidant activity was determined by two standard methods (as above mentioned). Total phenolic and flavonoid contents were estimated using Folin-Ciocalteu's phenol reagent and aluminum chloride colorimetric assay methods, respectively.
Results
(1) DPPH free radical scavenging ability assay
1. Free radical scavenging ability of ethanol extracts was higher than water extracts for all CHMs.
2. Ethanol extracts of Richardia scabra and Taxillus chinensis had similar ability to BHA, Anemarrhena asphodeloides had also stronger power than vitamin C.
3. The order of scavengingfree radical ability for water extracts were BHA> Rs> Tc> Vit.C ≈ Aa.
(2) Ferric reducing antioxidant power (FRAP) assay
The water extract of Taxillus chinensis had a strong reducing force that was due to the highest content of total phenols and total flavonoids (about the same as vitamin C)
(3) Total phenol content (TPC) assay
Water extracts of 4 CHMs had higher total phenol content than ethanol extracts, and the largest total phenol content found in Taxillus chinensis.
(4) Total flavonoid content (TFC) assay
The highest value of total flavonoid content showed in water extract of Taxillus chinensis and was twice as high as Richardia scabra.
Conclusion
The pH range of four CHMs extracts were 5.45 to 6.3 that is suitable added into skincare product. Taxillus chinensis is a potential source of antioxidants and serves as an effective free radical scavenger and/or inhibitor.
Chinese herbal medicine (CHMs) is widely used in Taiwan, and they are closely related to our life. A variety of Chinese herbal medicine has different effects, such as: Richardia scabra (Rs) for traditional folk gynecological medicine, has the effect of anti-inflammatory; Taxillus chinensis (Tc) can nourish the liver and kidneys, dredge the meridians, strengthen the muscles and bones; Arundo donax (Ad) and Anemarrhena asphodeloides (Aa) are commonly used to purge internal heat. In this study, we choose above mentioned four Chinese herbal medicines to carry out the biological evaluations of water and ethanol extracts of 4 CHMs by simple and widely popular method for screening free radical-scavenging ability and ferric reducing antioxidant power (FRAP). The objective of this study is to develop the new raw materials that can be used in cosmetic industry.
Methods
The antioxidant activity was determined by two standard methods (as above mentioned). Total phenolic and flavonoid contents were estimated using Folin-Ciocalteu's phenol reagent and aluminum chloride colorimetric assay methods, respectively.
Results
(1) DPPH free radical scavenging ability assay
1. Free radical scavenging ability of ethanol extracts was higher than water extracts for all CHMs.
2. Ethanol extracts of Richardia scabra and Taxillus chinensis had similar ability to BHA, Anemarrhena asphodeloides had also stronger power than vitamin C.
3. The order of scavengingfree radical ability for water extracts were BHA> Rs> Tc> Vit.C ≈ Aa.
(2) Ferric reducing antioxidant power (FRAP) assay
The water extract of Taxillus chinensis had a strong reducing force that was due to the highest content of total phenols and total flavonoids (about the same as vitamin C)
(3) Total phenol content (TPC) assay
Water extracts of 4 CHMs had higher total phenol content than ethanol extracts, and the largest total phenol content found in Taxillus chinensis.
(4) Total flavonoid content (TFC) assay
The highest value of total flavonoid content showed in water extract of Taxillus chinensis and was twice as high as Richardia scabra.
Conclusion
The pH range of four CHMs extracts were 5.45 to 6.3 that is suitable added into skincare product. Taxillus chinensis is a potential source of antioxidants and serves as an effective free radical scavenger and/or inhibitor.