Vitis Vinifera extract counteracts exposome aggressions in a 3D full-thickness human skin model exposed to different environmental stressors
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Presented by: Zhuo Cai
Vitis Vinifera extract counteracts exposome aggressions in a 3D full-thickness human skin model exposed to different environmental stressors
Zhuo Cai1, Yuan Lu1,Marion ALBOUY2, Sandrine HERAUD2, Amélie THEPOT2, Jsssica Wang1, Morgan DOS SANTOS2
2. LABSKIN CREATIONS, Edouard Herriot Hospital, 69003 LYON, France
Key words: V. vinifera, exposome, pollution, 3D skin model
Background
The skin is an essential barrier, protecting the body against the environment and its numerous pollutants and daily stresses. Several exposome aggressions, such as sunlight and pollution are known to affect the skin and can trigger molecular processes that accelerate premature skin aging through mechanisms including oxidative stress, inflammation, and impairment of skin functions. Self-induced factors such as diet, smoking and other miscellaneous factors, including lifestyle choices and use of cosmetic products, also play a significant role in potentiating skin aging.
Natural products such as plant extracts have been involved in the skin anti-oxidant and anti-aging cellular protection against environmental stress. Vitis vinifera regarded as an important medicinal plant and its main active polyphenol, resveratrol, have shown considerable antioxidant properties, besides possessing protective and therapeutic effects against various skin complications such as inflammation and wound healing.
In the present study, we aimed to study potential repairing properties conferred by V. vinifera extract in a 3D full-thickness human skin model exposed to individual stress including environmental pollutants, UVA and UVB radiations and detergent. In particular, we focused on the effect of the V. vinifera extract on epidermal barrier and cohesion, dermal extracellular matrix synthesis and anti-oxidant effects.
Experimental Results
To start with, all environmental stresses individually applied on our 3D skin model significantly affected both cellular and tissular functionalities. Comparatively to the unstressed control condition, our results demonstrated a significant increase of MMP-1, NQO1 and metallothionein expression and a decrease of type I collagen, filaggrin, claudin-1, ceramides and LCE-1A expression, in our 3D skin model either exposed to UVA and UVB radiations, urban dust or SDS.
In the UVA/UVB-exposed 3D skin model, V. vinifera extract significantly inhibited MMP-1 (75%, p=3.65E-5), NQO1 (72%, p=2.41E-10), and metallothionein (55%, p=1.79E-10). Collagen I, filaggrin, claudin-1, ceramides and LCE-1A expression significantly increased by 20% (p=0.0054), 110% (p=0.0045), 80% (p=2.96E-5), 155% (p=0.0025) and 1485% (p=0.0005), respectively.
In vitro study of V. vinifera on urban dust-exposed 3D skin model showed a significant increase of type I collagen, filaggrin, claudin-1 and LCE-1A expression by 29% (p=0,0033), 120% (p=1.51E-6), 91% (p=1.84E-5) and 1884% (p=1.70E-6), respectively. Conversely, V. vinifera decreased the expression by 62% (p=0.0024) and 77% (p=1.65E-6) of MMP-1 and NQO1, respectively.
Finally, V. vinifera applied on SDS irritant-treated 3D skin model demonstrated a significant decrease of NQO1 expression by 10% (p=0.009) and an increase of type I collagen expression by 52% (p=3.1300E-5).
Conclusion/Discussion
Overall, the present in vitro study demonstrated that application of V. vinifera extract on a 3D full-thickness human skin model exposed to different environmental aggressions prevented upregulation of oxidative markers by inhibiting NQO1 and metallothionein, improved skin barrier function by up-regulating filaggrin, LCE-1A, claudin-1 expression and also by stimulating extracellular matrix-related proteins such as type I collagen.
In conclusion, application of V. vinifera significantly counteracts the effects of exposome aggressions in an in vitro skin model, suggesting its protective effects against daily UV and pollutant exposure.