Lipid nanoparticles (LNPs) have a widely applied to pharmaceutical as drug delivery carrier. LNPs are currently spotlight as mRNA vaccines carrier. The LNPs are the self-assembly of lipids and could encapsulate the hydrophobic not only but also hydrophilic materials. LNPs have a micelle-like structure and are known to effective delivery the vaccine than classical lipid-based particles. In general, LNPs are composed of neutral phospholipids (1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)) and cholesterol to enhance the stability of lipid membrane and the negative lipids (1,2-dioleoyl-sn-glycero-3-phosphate (DOPA), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS)) and the positive lipids (dimethyldioctadecylammonium (DDA), 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP)). The charged lipids could be modified the structure of LNPs and surface characters. LNPs take advantages that enhancing the solubility, augment the permeability, bioavailability, and protection of the active ingredient. They also have high physical and biological stability unlike liposome. LNPs have been manufactured by various method such as microfluidics, thin film hydration, reverse-phase evaporation, and emulsion method. Among them, the microfluidic systems have been recently applied. The microfluidics uses the microchannels to control the aqueous phase and lipid phase and can change the conditions (flow rate, temperature, flow ratio) for optimization of LNPs. Microfluidics take the advantages of controlling the particle size, high reproducibility, and possible to continuous operation. Retinoids are defined as vitamin A or are chemically related to it. One of them, retinal, which is a form oxidized retinol, have more effective anti-wrinkle than retinol and led to increase dermal collagen synthesis. In this study, LNPs were used to encapsulate the retinal which is unstable under the oxygen environment. The LNPs were synthesized by using DOTAP as ionizable cationic lipid and as follow changing the flow rate and ratio. The synthesized LNPs were investigated using dynamic light scattering (DLS), cryo transmission electron microscope (Cryo-TEM), ultraviolet–visible (UV) spectrometer, and fourier-transform infrared spectroscopy (FT-IR). The particle size of LNPs were range of 50-1000nm and zeta potential were all positive charge. The LNPs encapsulated the retinal were successfully synthesized and stability enhanced. This study could apply for various fields such as drug delivery, food, and cosmetics.