The Effect and Analysis of Compound Polypeptide Firming Essence
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Presented by: Yu Yu
Introduction
Anti-aging, as the current mainstream skin care appeal, is getting more and more attention. Anti-aging products that claim to improve facial skin relaxation, promote firming, local lifting have a high market share and a wide range of consumer groups.
Compared with young skin, aging skin is dry, dull and shows some degree of laxity and local tissue sagging. According to research data, the skin elasticity value of the whole face of northern Chinese women aged 40-50 shows a downward trend with age, and the facial skin elasticity decreases rapidly after the age of 50. Improving skin elasticity and firmness is important for skin anti-aging.
Polypeptides have the advantages of biocompatibility, easy absorption, safety, good water solubility, low molecular weight, etc. At the same time, they have the functions of protecting, repairing damaged cells and promoting cell growth. Therefore, polypeptides have great development potential as anti-aging substances.
The objective of this study was to assess the firming improving effect of the compound polypeptide firming Essence.
Methods
In vitro evaluation
L929 mouse fibroblasts viability assay by MTT assay method and effects on collagen content.
Human test
A total of 30 Chinese women between 35~60 years old with sagging skin were enrolled, according to the Declaration of Helsinki, this test is voluntary by the subjects themselves, the purpose and possible risks of this test are informed before the test.
The left side is the control side, using the placebo, and the right side is the experimental side, using the compound polypeptide firming essence. Both side samples were applied on the face 2 times per day( morning and night)for 4 weeks. Elasticity data measurement, skin ultrasound and skin image acquisition were obtained once a week; Subjective satisfaction of volunteers were assessed after 4 weeks . Dermalab Series SkinLab Combo was used to measure elasticity data and get ultrasound Image. Images of all subjects were taken by VISIA .
Results
In vitro evaluation
1. After treatment of 0.5%, 0.1%, 0.05%, 0.01%, 0.005%, 0.001%essence, the cell viabilities were more than 85% completely, compared with that of the controll cells, it indicated the essence had no cytotoxic at the experimental concentration. It has the best effect of promoting cell proliferation under the condition of 0.001% concentration.
2. Compared with the cell control group, all the essence of 1%, 0.1%, 0.001% concentrations have a certain effect of promoting collagen production.
Human test
1. After 4 weeks, the increase in elasticity value on the experimental side is greater than the control side. The E value(Young´s elasticity modulus ) of experimental side increased by 10.87% (P<0.05), the VE value(Visco Elasticity) of experimental side increased by 7.50% (P<0.05), the R value(Skin retraction time) of experimental side reduced by 4.97% (P<0.05).
2. According to the data analyzed by VISIA, the improvement in pores value on the experimental side is greater than the control side. The Feature Counts of pores of experimental side reduced by 22.15% (P<0.05), the Absolute Scores of pores of experimental side reduced by 27.07% (P<0.05).
3. According to ultrasound Image, collagen intensity increased in more than 80% of volunteers.
4. More than 90% of volunteers thought their skin was firmer and more delicate.
Conclusions
The Compound Polypeptide Firming Essence has an excellent effect on improving skin elasticity and firmness, it also has a good effect of fine pores, shows good application prospects in the field of anti-aging skin care products.
Anti-aging, as the current mainstream skin care appeal, is getting more and more attention. Anti-aging products that claim to improve facial skin relaxation, promote firming, local lifting have a high market share and a wide range of consumer groups.
Compared with young skin, aging skin is dry, dull and shows some degree of laxity and local tissue sagging. According to research data, the skin elasticity value of the whole face of northern Chinese women aged 40-50 shows a downward trend with age, and the facial skin elasticity decreases rapidly after the age of 50. Improving skin elasticity and firmness is important for skin anti-aging.
Polypeptides have the advantages of biocompatibility, easy absorption, safety, good water solubility, low molecular weight, etc. At the same time, they have the functions of protecting, repairing damaged cells and promoting cell growth. Therefore, polypeptides have great development potential as anti-aging substances.
The objective of this study was to assess the firming improving effect of the compound polypeptide firming Essence.
Methods
In vitro evaluation
L929 mouse fibroblasts viability assay by MTT assay method and effects on collagen content.
Human test
A total of 30 Chinese women between 35~60 years old with sagging skin were enrolled, according to the Declaration of Helsinki, this test is voluntary by the subjects themselves, the purpose and possible risks of this test are informed before the test.
The left side is the control side, using the placebo, and the right side is the experimental side, using the compound polypeptide firming essence. Both side samples were applied on the face 2 times per day( morning and night)for 4 weeks. Elasticity data measurement, skin ultrasound and skin image acquisition were obtained once a week; Subjective satisfaction of volunteers were assessed after 4 weeks . Dermalab Series SkinLab Combo was used to measure elasticity data and get ultrasound Image. Images of all subjects were taken by VISIA .
Results
In vitro evaluation
1. After treatment of 0.5%, 0.1%, 0.05%, 0.01%, 0.005%, 0.001%essence, the cell viabilities were more than 85% completely, compared with that of the controll cells, it indicated the essence had no cytotoxic at the experimental concentration. It has the best effect of promoting cell proliferation under the condition of 0.001% concentration.
2. Compared with the cell control group, all the essence of 1%, 0.1%, 0.001% concentrations have a certain effect of promoting collagen production.
Human test
1. After 4 weeks, the increase in elasticity value on the experimental side is greater than the control side. The E value(Young´s elasticity modulus ) of experimental side increased by 10.87% (P<0.05), the VE value(Visco Elasticity) of experimental side increased by 7.50% (P<0.05), the R value(Skin retraction time) of experimental side reduced by 4.97% (P<0.05).
2. According to the data analyzed by VISIA, the improvement in pores value on the experimental side is greater than the control side. The Feature Counts of pores of experimental side reduced by 22.15% (P<0.05), the Absolute Scores of pores of experimental side reduced by 27.07% (P<0.05).
3. According to ultrasound Image, collagen intensity increased in more than 80% of volunteers.
4. More than 90% of volunteers thought their skin was firmer and more delicate.
Conclusions
The Compound Polypeptide Firming Essence has an excellent effect on improving skin elasticity and firmness, it also has a good effect of fine pores, shows good application prospects in the field of anti-aging skin care products.