Preparation and application of astaxanthin nanoemulsion freeze-dried powder with high stability
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Presented by: Yu Yu
Introduction: Astaxanthin, a type of carotenoid, exhibits a stronger antioxidant activity than vitamin E and β-carotene. It is widely used in medical treatment, cosmetics, health food and other industries. However, the utilization of astaxanthin is currently limited due to its low bioavailability, poor water-solubility and instability under adverse conditions (such as acidic environment, heat, light, etc.).
At present, traditional astaxanthin oil and soft capsule products still occupy the majority of the market, but astaxanthin formulations are becoming more and more abundant, such as micro/nano carriers, which improve the water solubility of astaxanthin. At the same time, the micro/nano carrier can overcome the keratin barrier and enhance the permeability of active ingredient.
However, it also has certain disadvantages, such as particle aggregation may still occur during long-term storage, which shortens the shelf life of astaxanthin products. And liquid products are prone to leakage and bacterial infection during transportation.
Therefore, this study aims to prepare freeze-dried astaxanthin nanoemulsion powder by freeze-drying technology, further improve the stability of astaxanthin nanoemulsion, and extend the shelf life of the product.
Method: In this paper, a high-stability freeze-dried astaxanthin nanoemulsion powder was prepared by the low-energy emulsification method and freeze-drying technology. By observing the appearance, particle size change and reconstitution time of the astaxanthin nanoemulsion after freeze-drying, the type and content of the freeze-dried protective agent were determined. And by measuring the retention rate of astaxanthin, the storage stability of freeze-dried astaxanthin nanoemulsion powder was studied.
Results: The astaxanthin nanoemulsion freeze-dried powder in this study has smooth surface, low viscosity, fast reconstitution rate, and has good protective effect on nanostructures, more importantly, it has high stability under the conditions of light and 40°C. When astaxanthin nanoemulsion was stored under light or 40℃ for one month, the retention rate of astaxanthin was almost zero. The retention rate of astaxanthin in freeze-dried powder was 68.45% when it was stored under light for 6 months. When stored at 40℃ for 6 months, the retention rate is 60.10%, which is significantly improved. After redissolution, the particle size of astaxanthin nanoemulsion only increased by 7nm. The freeze-dried powder protected the nanostructure and ensured the dispersion and stability of the nanoparticles in water after reconstitution.
Discussion and conclusion: In this study, astaxanthin nanoemulsion freeze-dried powder was prepared by low-energy emulsification and freeze-drying technology, which does not add preservatives and has high safety. The freeze-dried powder is easy to store and easy to transport, it greatly improves the retention rate of astaxanthin under light and high temperature conditions, and the problem of bacterial infection in the transportation of liquid products was overcome.
At present, traditional astaxanthin oil and soft capsule products still occupy the majority of the market, but astaxanthin formulations are becoming more and more abundant, such as micro/nano carriers, which improve the water solubility of astaxanthin. At the same time, the micro/nano carrier can overcome the keratin barrier and enhance the permeability of active ingredient.
However, it also has certain disadvantages, such as particle aggregation may still occur during long-term storage, which shortens the shelf life of astaxanthin products. And liquid products are prone to leakage and bacterial infection during transportation.
Therefore, this study aims to prepare freeze-dried astaxanthin nanoemulsion powder by freeze-drying technology, further improve the stability of astaxanthin nanoemulsion, and extend the shelf life of the product.
Method: In this paper, a high-stability freeze-dried astaxanthin nanoemulsion powder was prepared by the low-energy emulsification method and freeze-drying technology. By observing the appearance, particle size change and reconstitution time of the astaxanthin nanoemulsion after freeze-drying, the type and content of the freeze-dried protective agent were determined. And by measuring the retention rate of astaxanthin, the storage stability of freeze-dried astaxanthin nanoemulsion powder was studied.
Results: The astaxanthin nanoemulsion freeze-dried powder in this study has smooth surface, low viscosity, fast reconstitution rate, and has good protective effect on nanostructures, more importantly, it has high stability under the conditions of light and 40°C. When astaxanthin nanoemulsion was stored under light or 40℃ for one month, the retention rate of astaxanthin was almost zero. The retention rate of astaxanthin in freeze-dried powder was 68.45% when it was stored under light for 6 months. When stored at 40℃ for 6 months, the retention rate is 60.10%, which is significantly improved. After redissolution, the particle size of astaxanthin nanoemulsion only increased by 7nm. The freeze-dried powder protected the nanostructure and ensured the dispersion and stability of the nanoparticles in water after reconstitution.
Discussion and conclusion: In this study, astaxanthin nanoemulsion freeze-dried powder was prepared by low-energy emulsification and freeze-drying technology, which does not add preservatives and has high safety. The freeze-dried powder is easy to store and easy to transport, it greatly improves the retention rate of astaxanthin under light and high temperature conditions, and the problem of bacterial infection in the transportation of liquid products was overcome.