Probiotics are living bacteria that are intended to have health benefits into the body and skin. Good living microorganisms are consumed as yogurt of fermented foods for body. However, they are not able to apply to skin as cosmetics. Heat-killed lactobacillus is known as beneficial bacteria to strength the skin barrier. Homogenizer and microfludizer were used for stabilization of heat-killed bacteria including insoluble components into liposomes. The liposomes were consisted to 1-4% hydrogenated lecithin and
Physicohemical properties (particle size, zeta potential) of the liposomes were evaluated under various conditions of temperature (4, 25, 50 ℃) for one month, which results indicate their stability states.
The optimized liposome formulation was selected and its skin permeability was investigated using Franz diffusion cells. The liposomes provide enhanced delivery of the components of lactobacillus into the skin.
The cream containing probiotic liposomes improved the wrinkles around the eyes for 2 weeks.
These results suggest that the useful information for stabilization of insoluble heat-killed lactobacillus which strength skin barrier and anti-aging as cosmetics agents.