Ozonized glycerin (OG)-based cosmetic products lighten age spots on human facial skin
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Presented by: sayaka kojima
Background: Few cosmetic ingredients are shown to be able to safely remove or lighten facial dark spots once they have formed. OG has been reported to possess oxidation power and exhibit various biological activities such as antibacterial, antiviral and wound healing promotion.
Aims: This study is aimed to clarify the effects of OG on human skin, especially on age spots in the face.
Methods: OG serums (80 and 800 ppm) were mixed with melanin reagent in vitro for 4 weeks and then assessed for its ability to degrade the melanin. OG was also investigated its effect on gene expression of keratinocyte differentiation markers in vitro to explore the cell maturation. In clinical study for evaluation of effects of OG serums on melanin content in facial skin, 48 women were enrolled and measured for melanin content of age spots by a Mexameter at 4 and 8 weeks after daily twice application of them. Adverse events were monitored during the study.
Results: Both OG serums showed direct melanin degradation in a time-dependent manner, with significant effects observed as early as 6 hours. OG serum at 800 ppm showed higher activity than OG serum at 80 ppm, and the amount of melanin was decreased by about 40% on the day 14 of the mixing reaction. Differentiation marker studies using human keratinocytes showed that gene expression of involucrin and serine palmitoyltransferase was upregulated by OG, which was almost equivalent concentration to OG serum 80 ppm, suggesting that OGs can enhance turnover of the skin epidermis. In clinical study, OG serums 80 and 800 ppm showed larger decreases in melanin contents at 8 weeks compared to those at 4 weeks and their mean values of △melanin index were -16.7 and -15.2, respectively. Statistically significant differences were detected against respective controls. Number of subjects with a decrease in melanin index from baseline to 4 or 8 weeks increased in both OG serums 80 and 800 ppm, especially prominent at 8 weeks. There were no adverse events related to treatments of OG 80 and 800 ppm during the study.
Conclusion: The result indicated that applications of OG serums are safe and effective in lightening age spots on the facial skin.
Aims: This study is aimed to clarify the effects of OG on human skin, especially on age spots in the face.
Methods: OG serums (80 and 800 ppm) were mixed with melanin reagent in vitro for 4 weeks and then assessed for its ability to degrade the melanin. OG was also investigated its effect on gene expression of keratinocyte differentiation markers in vitro to explore the cell maturation. In clinical study for evaluation of effects of OG serums on melanin content in facial skin, 48 women were enrolled and measured for melanin content of age spots by a Mexameter at 4 and 8 weeks after daily twice application of them. Adverse events were monitored during the study.
Results: Both OG serums showed direct melanin degradation in a time-dependent manner, with significant effects observed as early as 6 hours. OG serum at 800 ppm showed higher activity than OG serum at 80 ppm, and the amount of melanin was decreased by about 40% on the day 14 of the mixing reaction. Differentiation marker studies using human keratinocytes showed that gene expression of involucrin and serine palmitoyltransferase was upregulated by OG, which was almost equivalent concentration to OG serum 80 ppm, suggesting that OGs can enhance turnover of the skin epidermis. In clinical study, OG serums 80 and 800 ppm showed larger decreases in melanin contents at 8 weeks compared to those at 4 weeks and their mean values of △melanin index were -16.7 and -15.2, respectively. Statistically significant differences were detected against respective controls. Number of subjects with a decrease in melanin index from baseline to 4 or 8 weeks increased in both OG serums 80 and 800 ppm, especially prominent at 8 weeks. There were no adverse events related to treatments of OG 80 and 800 ppm during the study.
Conclusion: The result indicated that applications of OG serums are safe and effective in lightening age spots on the facial skin.