Moisturizing and anti-wrinkle effect of Korean Natural citrion Junos Oil and bioconverted ethylhexyl Korean citron Junos oil
304
Presented by: Hye-Young Sung
Abstract
In this study, ethylhexyl bioconversion oil was prepared by applying Korean citron seed oil, a natural vegetable oil, and biocatalyst conversion technology, and the moisturizing and anti-wrinkle effects of each oil were confirmed.
As the number of consumers seeking eco-friendly materials increases, preference for natural vegetable oils is increasing, but there are limitations in application to cosmetic formulations due to the heavy feeling and stickiness of vegetable oils.
Naturally-derived vegetable oil contains more than 85% of tri-acylglycerol and 15% of the remaining di-acylglycerol and mono-acylglycerol among fatty acids, whereas bioconverted oil has almost no tri-acylglycerol, and contains di-acylglycerol and mono-acylglycerol. will occupy most of The prepared naturally-derived vegetable oil and bioconversion oil showed different properties and properties from each other. Compared to the naturally-derived vegetable oil, the ethylhexyl bioconversion oil had a lighter color and lower viscosity.
In addition, in this study, the moisturizing and anti-wrinkle effects of natural vegetable oil and ethylhexyl bioconversion oil were confirmed. Hyaluronic Acid (HA) and Filaggrin (FLG) production were used as indicators for moisturizing efficacy evaluation using keratinocytes (HaCaT cells), and pro-collagen synthesis amount using human fibroblasts (NHDF cells) for wrinkle improvement efficacy evaluation.
Methods
Preparation of natural oil and bio-conversion oil
The material of natural oil used in this experiment is the seeds of Yuja. Crude Yuja seed oil containing solids was obtained using the screw type oil pressed machine.
Bio-conversion yuja oil was manufactured by the principle of trans-esterification of 2-ethylhexnol though an enzymatic reaction. The molar ratio of natural yuja oil and 2-ethlyhexanol was 1:1. After the reaction, free fatty acid was removed through a distillation process to reduce odor.
Moisturizing effect
HaCaT cells were grown to high density in 24-well plates. Immediately before experiments, cells were washed two times with serum-free medium to completely remove HA accumulated during cell growth. Subsequently, HaCat cells were cultured with or without NYO for 24 hr. at the indicated time, supernatants were analyzed for HA or FLG using an enzyme-linked immunosorbent assay (ELISA) kit (Elabscience Biotechnology Co., LTD or Cusabio Biotech Co., LTD)
Procollagen contents effect
HDF cell culture conditions for 24 hrs. After incubation, the medium was replaced with 50 μg/ml medium containing the various concentration of PCP mixed with serum-free kit and the cells were cultured for 24 hrs. The procollagen contents in culture supernatant were measured using Procollagen type I-c-peptodie (PIP) ELASA kit according to manufacturer’s manual. Results were normalized on total protein content of the supernatant.
Moisturizing human efficacy evaluation
Measure skin moisture using Corneometer (CM825, Courage+ Khazaka electronic GmbH, Germany). The test was conducted the number of test persons was 20, and the test site was the forearm inside the arm. 2μL/cm² of the sample was applied to the selected test site (1.5cm X 1.5cm) of the forearm, and measurements were taken immediately after application, after 4 hours, and after 8 hours
Evaluation of human efficacy for improving eye wrinkles
The degree of wrinkles around the eyes was measured using ANTERA 3D (Miravexn Limeted, Ireland). The test was conducted the number of test persons was 44, and the test site was the face (around the eyes). The test subjects took a drop or two of the sample on the palm of the hand to make it similar to body temperature, applied it to the face, and then tapped it to absorb it. Apply a sufficient amount twice a day in the morning and evening.
Results
In the case of Korean citron oil and ethylhexyl citron oil, HA increased by sample treatment, but FLG silver increased insignificantly. Both increases in pro-collagen were weak. However, in clinical trials, both citron oil and ethylhexyl citron oil showed statistically significantly higher moisture improvement and moisture retention effects than the control group after 4 hours and 8 hours without adverse skin reactions. The overall size and depth of both oils also confirmed a statistically significant level of improvement in the wrinkles around the eyes after 4 weeks of sample application.
Discussion and Conclusion
In this study, the feeling of use was improved when applied to the skin through biocatalytic conversion technology, and it is expected that it will be continuously expanded as a cosmetic material by confirming the moisturizing and anti-wrinkle effects.
In this study, ethylhexyl bioconversion oil was prepared by applying Korean citron seed oil, a natural vegetable oil, and biocatalyst conversion technology, and the moisturizing and anti-wrinkle effects of each oil were confirmed.
As the number of consumers seeking eco-friendly materials increases, preference for natural vegetable oils is increasing, but there are limitations in application to cosmetic formulations due to the heavy feeling and stickiness of vegetable oils.
Naturally-derived vegetable oil contains more than 85% of tri-acylglycerol and 15% of the remaining di-acylglycerol and mono-acylglycerol among fatty acids, whereas bioconverted oil has almost no tri-acylglycerol, and contains di-acylglycerol and mono-acylglycerol. will occupy most of The prepared naturally-derived vegetable oil and bioconversion oil showed different properties and properties from each other. Compared to the naturally-derived vegetable oil, the ethylhexyl bioconversion oil had a lighter color and lower viscosity.
In addition, in this study, the moisturizing and anti-wrinkle effects of natural vegetable oil and ethylhexyl bioconversion oil were confirmed. Hyaluronic Acid (HA) and Filaggrin (FLG) production were used as indicators for moisturizing efficacy evaluation using keratinocytes (HaCaT cells), and pro-collagen synthesis amount using human fibroblasts (NHDF cells) for wrinkle improvement efficacy evaluation.
Methods
Preparation of natural oil and bio-conversion oil
The material of natural oil used in this experiment is the seeds of Yuja. Crude Yuja seed oil containing solids was obtained using the screw type oil pressed machine.
Bio-conversion yuja oil was manufactured by the principle of trans-esterification of 2-ethylhexnol though an enzymatic reaction. The molar ratio of natural yuja oil and 2-ethlyhexanol was 1:1. After the reaction, free fatty acid was removed through a distillation process to reduce odor.
Moisturizing effect
HaCaT cells were grown to high density in 24-well plates. Immediately before experiments, cells were washed two times with serum-free medium to completely remove HA accumulated during cell growth. Subsequently, HaCat cells were cultured with or without NYO for 24 hr. at the indicated time, supernatants were analyzed for HA or FLG using an enzyme-linked immunosorbent assay (ELISA) kit (Elabscience Biotechnology Co., LTD or Cusabio Biotech Co., LTD)
Procollagen contents effect
HDF cell culture conditions for 24 hrs. After incubation, the medium was replaced with 50 μg/ml medium containing the various concentration of PCP mixed with serum-free kit and the cells were cultured for 24 hrs. The procollagen contents in culture supernatant were measured using Procollagen type I-c-peptodie (PIP) ELASA kit according to manufacturer’s manual. Results were normalized on total protein content of the supernatant.
Moisturizing human efficacy evaluation
Measure skin moisture using Corneometer (CM825, Courage+ Khazaka electronic GmbH, Germany). The test was conducted the number of test persons was 20, and the test site was the forearm inside the arm. 2μL/cm² of the sample was applied to the selected test site (1.5cm X 1.5cm) of the forearm, and measurements were taken immediately after application, after 4 hours, and after 8 hours
Evaluation of human efficacy for improving eye wrinkles
The degree of wrinkles around the eyes was measured using ANTERA 3D (Miravexn Limeted, Ireland). The test was conducted the number of test persons was 44, and the test site was the face (around the eyes). The test subjects took a drop or two of the sample on the palm of the hand to make it similar to body temperature, applied it to the face, and then tapped it to absorb it. Apply a sufficient amount twice a day in the morning and evening.
Results
In the case of Korean citron oil and ethylhexyl citron oil, HA increased by sample treatment, but FLG silver increased insignificantly. Both increases in pro-collagen were weak. However, in clinical trials, both citron oil and ethylhexyl citron oil showed statistically significantly higher moisture improvement and moisture retention effects than the control group after 4 hours and 8 hours without adverse skin reactions. The overall size and depth of both oils also confirmed a statistically significant level of improvement in the wrinkles around the eyes after 4 weeks of sample application.
Discussion and Conclusion
In this study, the feeling of use was improved when applied to the skin through biocatalytic conversion technology, and it is expected that it will be continuously expanded as a cosmetic material by confirming the moisturizing and anti-wrinkle effects.