Identification of the molecular-level moisturizing effect on the entire skin layer of mixture composed of fermented seaweeds extract from Jeju island.
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Presented by: Soyoun Lee
Water is absolutely essential for the normal functioning of the skin and the loss of water from the skin must be carefully regulated. Otherwise, various skin problems such as dry skin, itchiness, and damage to skin tissue may occur, as well as reduced skin elasticity and wrinkles, so moisturizing is the most basic and essential function in cosmetics. This study was conducted to develop a new ingredient with moisturizing and water-loss prevention functions at the molecular-level to fundamentally prevent and solve the problem of lack of water in the skin.
In this study, we prepared a new moisturizing ingredient fermented with seaweed extracts from Jeju containing Gelidium Amansii, Hizikia Fusiforme, Codium Fragile, Ecklonia Cava, Sargassum Fulvellum. We investigated the molecular-level moisturizing effect of the seaweed extracts for entire skin layer in dermal fibroblasts and keratinocytes. The moisturizing activity was determined by Aquaporins (AQPs) and hyaluronic acid synthases (HASs) expression level. AQP1, 3, 9 and HAS1, 2, 3 mRNA level were measured by qRT-PCR. AQP1,3,9 and Hyaluronic acid (HA) protein level were evaluated by blotting analysis and immunocytochemistry. To confirm that the seaweed extracts actually acts on the entire skin layer, the expression level of AQPs in 3D full thickness skin model was evaluated using the immunostaining method. In addition, Strengthening the skin barrier activity was determined by thigh junction protein (Claudin, Occludin, Zo-1) expression level by qRT PCR and immunostaining method in keratinocytes. Clinical tests of skin moisture were performed using cosmetics containing the seaweed extracts.
The seaweed extracts induced the upregulation of different types of AQPs and HASs mRNA expression by the skin layer. In keratinocytes, the cells that make up the epidermis, AQP3 mRNA and protein levels were higher than those of AQP1 and AQP9. On the other hand, in dermal fibroblasts, the mRNA and protein level of AQP9 were increased compared to those of AQP1 and AQP3. The results were also confirmed in the 3D full thickness skin model. Likewise, HAS1, 2, 3 mRNA level was increased by the seaweed extract, HAS1 was increased more in epidermal cells and HAS2 and 3 more than in dermal cells. As the expression level of HAS, hyaluronic acid (HA) synthesis level in the skin cells was increased. Claudin, Occludin, Zo-1 mRNA and protein expression also increased by the seaweed extracts. Furthermore, after skin application of cosmetics containing the seaweed extracts, skin hydration was improved and moisturizing lasted for 24h in vivo.
The seaweed extracts found in the sea of Jeju has a moisturizing effect by promoting AQPs and HAS differently for each skin layer. These factors contribute to moisturizing at the molecular-level. And it promoted expression of tight junction proteins in vitro, and improved skin hydration in vivo. Therefore, the new seawedd extracts was shown to have value as a powerful moisturizing ingredient.
In this study, we prepared a new moisturizing ingredient fermented with seaweed extracts from Jeju containing Gelidium Amansii, Hizikia Fusiforme, Codium Fragile, Ecklonia Cava, Sargassum Fulvellum. We investigated the molecular-level moisturizing effect of the seaweed extracts for entire skin layer in dermal fibroblasts and keratinocytes. The moisturizing activity was determined by Aquaporins (AQPs) and hyaluronic acid synthases (HASs) expression level. AQP1, 3, 9 and HAS1, 2, 3 mRNA level were measured by qRT-PCR. AQP1,3,9 and Hyaluronic acid (HA) protein level were evaluated by blotting analysis and immunocytochemistry. To confirm that the seaweed extracts actually acts on the entire skin layer, the expression level of AQPs in 3D full thickness skin model was evaluated using the immunostaining method. In addition, Strengthening the skin barrier activity was determined by thigh junction protein (Claudin, Occludin, Zo-1) expression level by qRT PCR and immunostaining method in keratinocytes. Clinical tests of skin moisture were performed using cosmetics containing the seaweed extracts.
The seaweed extracts induced the upregulation of different types of AQPs and HASs mRNA expression by the skin layer. In keratinocytes, the cells that make up the epidermis, AQP3 mRNA and protein levels were higher than those of AQP1 and AQP9. On the other hand, in dermal fibroblasts, the mRNA and protein level of AQP9 were increased compared to those of AQP1 and AQP3. The results were also confirmed in the 3D full thickness skin model. Likewise, HAS1, 2, 3 mRNA level was increased by the seaweed extract, HAS1 was increased more in epidermal cells and HAS2 and 3 more than in dermal cells. As the expression level of HAS, hyaluronic acid (HA) synthesis level in the skin cells was increased. Claudin, Occludin, Zo-1 mRNA and protein expression also increased by the seaweed extracts. Furthermore, after skin application of cosmetics containing the seaweed extracts, skin hydration was improved and moisturizing lasted for 24h in vivo.
The seaweed extracts found in the sea of Jeju has a moisturizing effect by promoting AQPs and HAS differently for each skin layer. These factors contribute to moisturizing at the molecular-level. And it promoted expression of tight junction proteins in vitro, and improved skin hydration in vivo. Therefore, the new seawedd extracts was shown to have value as a powerful moisturizing ingredient.