The anti-aging effect of COLinus, human collagen-derived peptides, in vitro and ex-vivo study
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Presented by: So Hun Lee
The anti-aging effect of COLinus, human collagen-derived peptides, in vitro and ex-vivo study
So Hun Lee1, Byung Kuk Kim1, Ha Yeon Kim1, Jae Seok Lee1, Kang Hyuk Lee1, Byung Ho Shin2, Da Yeong NAM2 and Song Seok Shin1
1Life Science R&D Center, HYUNDAI BIOLAND Co., Ltd., South Korea
2Korean Skin Research Center (KSRC), South Korea
Abstract
Introduction:
As life expectancy increases, reducing visible signs of skin aging caused by various factors such as endogenous (genetic and hormonal factors) and exogenous (UV irradiation, continuous environmental stimuli, food and lifestyle) has become a major issue. There are several phenomena in skin aging, but the reduction of collagen synthesis and regeneration in human skin is a major feature of skin aging.
In previous studies, collagen derived from land and marine animals has been applied to cosmetics for various purposes. However, the collagen may have skin irritation issues such as allergies, and safety has not been secured. Therefore, we conducted a study to improve the skin condition by developing COLinus, human collagen-derived peptides with no skin irritation issue and high safety.
Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cell cytotoxicity and proliferation. Besides, a photograph was taken with a microscope through a scratch assay for wound healing in human dermal fibroblasts. In addition, real-time reverse transcription PCR (RT-PCR) and western blotting were performed to investigate the collagen and Elastin mRNA expression, and collagen protein expression in human dermal fibroblasts, respectively.
Results: The MTT assay revealed that COLinus was no significant cytotoxicity in human dermal fibroblasts. COLinus improved cell proliferation and wound healing in human dermal fibroblasts. In addition, COLinus markedly increased mRNA expression and protein expression of collagen type 1, collagen type 3 and Elastin. Moreover, as a result of performing a skin permeation test on ex vivo human cadaver skin, it was confirmed that the COLinus penetrated the human skin.
Discussion and Conclusion: The findings of this study confirmed that COLinus was well absorbed into the human skin and improved cell proliferation, cell migration, collagen synthesis as well as elastin synthesis in human dermal fibroblasts. In Conclusion, COLinus, human collagen-derived peptides, can be used as a future anti-aging cosmetic ingredient for improving skin conditions.
So Hun Lee1, Byung Kuk Kim1, Ha Yeon Kim1, Jae Seok Lee1, Kang Hyuk Lee1, Byung Ho Shin2, Da Yeong NAM2 and Song Seok Shin1
1Life Science R&D Center, HYUNDAI BIOLAND Co., Ltd., South Korea
2Korean Skin Research Center (KSRC), South Korea
Abstract
Introduction:
As life expectancy increases, reducing visible signs of skin aging caused by various factors such as endogenous (genetic and hormonal factors) and exogenous (UV irradiation, continuous environmental stimuli, food and lifestyle) has become a major issue. There are several phenomena in skin aging, but the reduction of collagen synthesis and regeneration in human skin is a major feature of skin aging.
In previous studies, collagen derived from land and marine animals has been applied to cosmetics for various purposes. However, the collagen may have skin irritation issues such as allergies, and safety has not been secured. Therefore, we conducted a study to improve the skin condition by developing COLinus, human collagen-derived peptides with no skin irritation issue and high safety.
Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cell cytotoxicity and proliferation. Besides, a photograph was taken with a microscope through a scratch assay for wound healing in human dermal fibroblasts. In addition, real-time reverse transcription PCR (RT-PCR) and western blotting were performed to investigate the collagen and Elastin mRNA expression, and collagen protein expression in human dermal fibroblasts, respectively.
Results: The MTT assay revealed that COLinus was no significant cytotoxicity in human dermal fibroblasts. COLinus improved cell proliferation and wound healing in human dermal fibroblasts. In addition, COLinus markedly increased mRNA expression and protein expression of collagen type 1, collagen type 3 and Elastin. Moreover, as a result of performing a skin permeation test on ex vivo human cadaver skin, it was confirmed that the COLinus penetrated the human skin.
Discussion and Conclusion: The findings of this study confirmed that COLinus was well absorbed into the human skin and improved cell proliferation, cell migration, collagen synthesis as well as elastin synthesis in human dermal fibroblasts. In Conclusion, COLinus, human collagen-derived peptides, can be used as a future anti-aging cosmetic ingredient for improving skin conditions.