A study on the use of Chamaecyparis obtuse leaves extract as a natural cosmetic ingredient by subcritical water extraction
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Presented by: minjeong kim
A study on the use of Chamaecyparis obtuse leaves extract as a natural cosmetic ingredient by subcritical water extraction
Min Jeong Kim1, Ji Young Moon1, Jung Eun Kim2, Nam Ho Lee2 and Jeong Mi Kim1
1 UCL Co. Ltd., Central Research Institute, Jeju, Republic of Korea
2Department of Chemistry and Cosmetics, Jeju National University, Republic of Korea
Key words: Subcritical water extraction; Chamaecyparis obtusa; Antioxidant, Anti-inflammatory, , Antibacterial
Abstract
Introduction: The purpose of this study is to develop cosmetics ingredient using subcritical water extraction (SWE) with Chamaecyparis obtusa leaves originated from Jeju-island and comparison of other methods of extraction. This SWE use only water and could extract active material without chemical solvent which is harmful to human’s skin. In this study the antioxidant, anti-inflammatory and antibacterial effects of Chamaecyparis obtusa extract of Jeju island native plant using SWE was confirmed.
Methods: Ground Chamaecyparis obtusa leaves ( 1.2g ) were subjected to subcritical water extraction. The extraction efficiencies of different temperatures ( 120, 135, 150 ℃ ), and pressures ( 60, 80 bar ) were investigated. The identification of components was carried out using HPLC analysis. And efficacy evaluation of each extract was measured by DPPH scavenging ability for antioxidant, NO inhibition for anti-inflammatory, and paper disc diffusion method for antibacterial.
Results: Subcritical water extraction from Chamaecyparis obtusa leaves exhibited the highest extraction efficiency at temperature 135 ℃, pressure 80 bar, flow rate 1 ml min-1, extract time 15min. At these optimum extraction parameters, the maximum yield 29.1%. As a result of confirming the antioxidant effect under optimal conditions, it exhibited a DPPH radical scavenging effect of about 80% when treated with 400 ug/mL, and an inhibitory effect of about 52% when treated with a concentration of 20 ug/mL. In addition, as a result of confirming the size of the transparent area for antibacterial activity through the reduction diffusion method, it was confirmed that the effect was shown on the skin-related strains Staphylococcus epidermidis and Corynebacteriume acnes. And based on HPLC results of SWE extract was found quercitrin 5.65 mg/g, catechin 8.89mg/g.
Conclusion: In conclusion, this study demonstrated that the Chamaecyparis obtusa leaves extract inhibitory effects of inflammatory mediator, antioxidant effects, and antibacterial effects. Moreover, the extraction process using subcritical extraction equipment is relatively shorter than the extraction time using general mass production equipment. It is considered to be an economical and efficient method for extraction. Based on the results of this study, it was possible to confirm the potential of Chamaecyparis obtuse leaves extract as a natural cosmetic ingredient by subcritical water extraction.
Min Jeong Kim1, Ji Young Moon1, Jung Eun Kim2, Nam Ho Lee2 and Jeong Mi Kim1
1 UCL Co. Ltd., Central Research Institute, Jeju, Republic of Korea
2Department of Chemistry and Cosmetics, Jeju National University, Republic of Korea
Key words: Subcritical water extraction; Chamaecyparis obtusa; Antioxidant, Anti-inflammatory, , Antibacterial
Abstract
Introduction: The purpose of this study is to develop cosmetics ingredient using subcritical water extraction (SWE) with Chamaecyparis obtusa leaves originated from Jeju-island and comparison of other methods of extraction. This SWE use only water and could extract active material without chemical solvent which is harmful to human’s skin. In this study the antioxidant, anti-inflammatory and antibacterial effects of Chamaecyparis obtusa extract of Jeju island native plant using SWE was confirmed.
Methods: Ground Chamaecyparis obtusa leaves ( 1.2g ) were subjected to subcritical water extraction. The extraction efficiencies of different temperatures ( 120, 135, 150 ℃ ), and pressures ( 60, 80 bar ) were investigated. The identification of components was carried out using HPLC analysis. And efficacy evaluation of each extract was measured by DPPH scavenging ability for antioxidant, NO inhibition for anti-inflammatory, and paper disc diffusion method for antibacterial.
Results: Subcritical water extraction from Chamaecyparis obtusa leaves exhibited the highest extraction efficiency at temperature 135 ℃, pressure 80 bar, flow rate 1 ml min-1, extract time 15min. At these optimum extraction parameters, the maximum yield 29.1%. As a result of confirming the antioxidant effect under optimal conditions, it exhibited a DPPH radical scavenging effect of about 80% when treated with 400 ug/mL, and an inhibitory effect of about 52% when treated with a concentration of 20 ug/mL. In addition, as a result of confirming the size of the transparent area for antibacterial activity through the reduction diffusion method, it was confirmed that the effect was shown on the skin-related strains Staphylococcus epidermidis and Corynebacteriume acnes. And based on HPLC results of SWE extract was found quercitrin 5.65 mg/g, catechin 8.89mg/g.
Conclusion: In conclusion, this study demonstrated that the Chamaecyparis obtusa leaves extract inhibitory effects of inflammatory mediator, antioxidant effects, and antibacterial effects. Moreover, the extraction process using subcritical extraction equipment is relatively shorter than the extraction time using general mass production equipment. It is considered to be an economical and efficient method for extraction. Based on the results of this study, it was possible to confirm the potential of Chamaecyparis obtuse leaves extract as a natural cosmetic ingredient by subcritical water extraction.