Evaluation of the anti-aging and skin rejuvenation effect of a novel facial cream containing small synthetic peptide which to unlock TGF-β potential
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Presented by: Ziyan Zhou
Introduction:
In recent years bioactive peptides have established themselves as cornerstone ingredients in skin care, mainly for anti-aging cosmetics. Transforming growth factor beta (TGF-β) as a role model for peptide design and the gateway to skin rejuvenation. This study is about a design a new peptide to unlock TGF-β potential with skin rejuvenation and evaluation the anti-aging effect of the facial cream containing the designed peptide.
Methods:
In-vitro LAP-TGF-β activation, the samples were tested with TGF-β Emax immunoassay system (promega). TGF-β and the peptide suppress pro-inflammatory factors (TNF-α, MMP-1, and MMP-3 and IL-8) in human normal keratinocytes. Keratinocyte culture and treatment phorbol 12-myristate 13-acetate (PMA) experiments were test. Quantitation of cytokines and matrix metalloproteinases (MMPs) were quantified from supernatant of cell cultures with a multiplex bead array system (Luminex 100 xMAP) using the Procarta Cytokine Assay Kit (Panomics, Fremont USA).
Clinical evaluation with 36 female subjects is to evaluate the anti-wrinkle effect of several products versus placebo. Study of the variations, directly in vivo, of the cutaneous relief parameters on crow’s feet and under eyes wrinkles (average roughness Ra, maximum amplitude Rt and average relief Rz) using DermaTOP (EOTECH - France). Illustration of the visual expected effect by realisation of macrophotographs (some of them were sent to NEWTONE Technologies ® for further analysis: dark circles).
Results:
The result of the in-vitro shows a significant higher time-dependent increase release of TGF-β from LAP-TGF-β complex after incubation with the peptide than control group. TGF-β and the peptide always strongly in habited up-regulation of TNF-α, MMP-1, and MMP-3 and IL-8 expression
The clinical evaluation result shows a significantly anti-wrinkle effect within 7 days, and smooth fine lines effect within 14 days.
Discussion and Conclusion:
The small synthetic peptide showed an unlock TGF-β potential function, and suppress pro-inflammatory factors (TNF-α, MMP-1, and MMP-3 and IL-8) in human normal keratinocytes. In clinical testing, the cream containing the peptide showed a significant smoothing and anti-wrinkle efficacy on crow’s feet and underneath eye after 28 days twice-daily application.
In recent years bioactive peptides have established themselves as cornerstone ingredients in skin care, mainly for anti-aging cosmetics. Transforming growth factor beta (TGF-β) as a role model for peptide design and the gateway to skin rejuvenation. This study is about a design a new peptide to unlock TGF-β potential with skin rejuvenation and evaluation the anti-aging effect of the facial cream containing the designed peptide.
Methods:
In-vitro LAP-TGF-β activation, the samples were tested with TGF-β Emax immunoassay system (promega). TGF-β and the peptide suppress pro-inflammatory factors (TNF-α, MMP-1, and MMP-3 and IL-8) in human normal keratinocytes. Keratinocyte culture and treatment phorbol 12-myristate 13-acetate (PMA) experiments were test. Quantitation of cytokines and matrix metalloproteinases (MMPs) were quantified from supernatant of cell cultures with a multiplex bead array system (Luminex 100 xMAP) using the Procarta Cytokine Assay Kit (Panomics, Fremont USA).
Clinical evaluation with 36 female subjects is to evaluate the anti-wrinkle effect of several products versus placebo. Study of the variations, directly in vivo, of the cutaneous relief parameters on crow’s feet and under eyes wrinkles (average roughness Ra, maximum amplitude Rt and average relief Rz) using DermaTOP (EOTECH - France). Illustration of the visual expected effect by realisation of macrophotographs (some of them were sent to NEWTONE Technologies ® for further analysis: dark circles).
Results:
The result of the in-vitro shows a significant higher time-dependent increase release of TGF-β from LAP-TGF-β complex after incubation with the peptide than control group. TGF-β and the peptide always strongly in habited up-regulation of TNF-α, MMP-1, and MMP-3 and IL-8 expression
The clinical evaluation result shows a significantly anti-wrinkle effect within 7 days, and smooth fine lines effect within 14 days.
Discussion and Conclusion:
The small synthetic peptide showed an unlock TGF-β potential function, and suppress pro-inflammatory factors (TNF-α, MMP-1, and MMP-3 and IL-8) in human normal keratinocytes. In clinical testing, the cream containing the peptide showed a significant smoothing and anti-wrinkle efficacy on crow’s feet and underneath eye after 28 days twice-daily application.