The study of mRNA expression, skin hydration, shrink pores and smooth skin function of Lens esculenta seed extract in cosmetics
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Presented by: Fan Yang
Introduction:
With the increase of age, the skin become grain, and the pores become thicker. During age-related skin slackening, the pore walls slacken and widen, supported by the epidermis and the dermis. The skin microrelief, defined by the orientation and the depth of the furrows, also contributes to pore distortion and age. Pore dilation, which leading to accumulation of nucleated cells around the pores, also seems to be related to an abnormal accelerated keratinization process.
This study is to evaluation the effect of the lens esculenta seed extract to restores the normal keratinization process, stimulate the expression of collagen I and reinforcing the pore wall support structures. And it is also researching the extract’s function of reduced nucleated cells and limited pore wall slackening. The clinical efficacy shown the actives function in formulation
Methods:
Evaluation of promoting effects on mRNA expression of genes related to skin barrier function，Keratinocytes were incubated with a test sample and gene expression levels of Transglutaminase 1 and Involucrin (IVL) were analyzed by real-time RT-PCR. Staining of nucleated cells is to assess in vivo the effect of active formulated in emulsiion on the keratinization process on the cheeks in comparison with placebo. The skin hydration evaluated by corneometer CM825. A four week Clinical testing was conducted on 30 chinese female subjects with pore and wrinkle by VISIA and Primos, and professional dermatologists evaluation.
Results:
Tested at 1% on normal human keratinocytes, Lens esculenta seed extract significantly increased the expression of mRNAs coding for transglutaminase 1 by 34%, involucrin by 27%. thus, favors keratinocyte differentiation. Lens esculenta seed extract significantly reduces the number of nucleated cells located around pores by 20.8% (p=0.0407).
The sample with 3% Lens esculenta seed extract produced good clinical improvements on skin hydration, shrink pore and tighten skin. The moisture content significant increase 61.80% after 1 hour sample used. In promos testing, the pore become shrinker after 56D treatment than before. The clinical dermatologist evaluation showed increase of 34.75% in skin fineness and smooth.
Discussion and Conclusion:
In summary, Lens esculenta seed extract has a significant increase of mRNA expression which help to shrink pores. In a clinical efficacy trail the sample contain 3% Lens esculenta seed extract shows good improvement in shrink pores and skin smooth function.
With the increase of age, the skin become grain, and the pores become thicker. During age-related skin slackening, the pore walls slacken and widen, supported by the epidermis and the dermis. The skin microrelief, defined by the orientation and the depth of the furrows, also contributes to pore distortion and age. Pore dilation, which leading to accumulation of nucleated cells around the pores, also seems to be related to an abnormal accelerated keratinization process.
This study is to evaluation the effect of the lens esculenta seed extract to restores the normal keratinization process, stimulate the expression of collagen I and reinforcing the pore wall support structures. And it is also researching the extract’s function of reduced nucleated cells and limited pore wall slackening. The clinical efficacy shown the actives function in formulation
Methods:
Evaluation of promoting effects on mRNA expression of genes related to skin barrier function，Keratinocytes were incubated with a test sample and gene expression levels of Transglutaminase 1 and Involucrin (IVL) were analyzed by real-time RT-PCR. Staining of nucleated cells is to assess in vivo the effect of active formulated in emulsiion on the keratinization process on the cheeks in comparison with placebo. The skin hydration evaluated by corneometer CM825. A four week Clinical testing was conducted on 30 chinese female subjects with pore and wrinkle by VISIA and Primos, and professional dermatologists evaluation.
Results:
Tested at 1% on normal human keratinocytes, Lens esculenta seed extract significantly increased the expression of mRNAs coding for transglutaminase 1 by 34%, involucrin by 27%. thus, favors keratinocyte differentiation. Lens esculenta seed extract significantly reduces the number of nucleated cells located around pores by 20.8% (p=0.0407).
The sample with 3% Lens esculenta seed extract produced good clinical improvements on skin hydration, shrink pore and tighten skin. The moisture content significant increase 61.80% after 1 hour sample used. In promos testing, the pore become shrinker after 56D treatment than before. The clinical dermatologist evaluation showed increase of 34.75% in skin fineness and smooth.
Discussion and Conclusion:
In summary, Lens esculenta seed extract has a significant increase of mRNA expression which help to shrink pores. In a clinical efficacy trail the sample contain 3% Lens esculenta seed extract shows good improvement in shrink pores and skin smooth function.