Paeonia Suffruticosa root bark extract in combination with the -MSH biomimetic peptide Greyverse™ stimulates pigmentation in human hair follicles.
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Presented by: David Broadley
Abstract
Introduction:
Canities is an age-linked loss of bulb melanocyte and hair follicle (HF) pigmentation. Canities can significantly alter the physical appearance of a person and negatively impact their well-being, which drives the search and demand for new anti-greying agents.
Our preliminary studies revealed that Paeonia Suffruticosa root bark extract (PS) in combination with the αMSH biomimetic synthetic peptide Greyverse™ synergically induces mitochondrial E3 ligase (MITOL) and melanogenesis in human melanocytes in vitro. αMSH is a pro-pigmentary hormone of the melanocortin family, which stimulates melanogenesis through its target receptor, MC1. Gv is an established promoter of HF melanogenesis with redox properties. This study investigated the additive effects of PS and Gv on hair pigmentation ex vivo.
Methods: In an experimental research study, we investigated if PS and GV modulate human HF melanogenesis. HFs were cultured in serum-free medium, supplemented with PS and/or Gv for four days, then assessed for melanin content, protein expression and enzymatic changes associated to melanogenesis. By immuno-histomorphometry, we quantified the hair matrix expression of the premelanosome protein GP100 and the activity of Tyrosinase, which is the rate-limiting enzyme of melanin synthesis. Further, the Fontana Masson stain was used to confirm any changes in melanin production in hair matrix melanocytes.
Results:
Quantitative immune-histomorphometry revealed that PS and Gv cotreatment led to an additive increase in melanin content (p<0.05) that derived from a synergic increase in Gp100 expression (p<0.05), an increase in tyrosinase activity, and αMSH and TYRP expression within the hair follicle pigmentary unit.
Discussion and Conclusion:
In conclusion, we provide evidence that PS stimulates Gp100 expression and thereby melanogensis in human hair follicles. PS in combination with Gv showed synergic effects in promoting hair pigmentation, primarily through melanosome maturation. Thus, this combinational treatment deserves further pre-clinical and clinical exploration for the reversal of hair greying. In a clinical study with male volunteers, Greyverse™ decreased the overall whiteness of hair and darkened grey hair after three months of treatment leading to an overall reduction of 30% in grey density. It will be important to assess if PS in combination with Gv can further reduce the percentage of canities, similar to our results in ex vivo cultured human HFs.
Introduction:
Canities is an age-linked loss of bulb melanocyte and hair follicle (HF) pigmentation. Canities can significantly alter the physical appearance of a person and negatively impact their well-being, which drives the search and demand for new anti-greying agents.
Our preliminary studies revealed that Paeonia Suffruticosa root bark extract (PS) in combination with the αMSH biomimetic synthetic peptide Greyverse™ synergically induces mitochondrial E3 ligase (MITOL) and melanogenesis in human melanocytes in vitro. αMSH is a pro-pigmentary hormone of the melanocortin family, which stimulates melanogenesis through its target receptor, MC1. Gv is an established promoter of HF melanogenesis with redox properties. This study investigated the additive effects of PS and Gv on hair pigmentation ex vivo.
Methods: In an experimental research study, we investigated if PS and GV modulate human HF melanogenesis. HFs were cultured in serum-free medium, supplemented with PS and/or Gv for four days, then assessed for melanin content, protein expression and enzymatic changes associated to melanogenesis. By immuno-histomorphometry, we quantified the hair matrix expression of the premelanosome protein GP100 and the activity of Tyrosinase, which is the rate-limiting enzyme of melanin synthesis. Further, the Fontana Masson stain was used to confirm any changes in melanin production in hair matrix melanocytes.
Results:
Quantitative immune-histomorphometry revealed that PS and Gv cotreatment led to an additive increase in melanin content (p<0.05) that derived from a synergic increase in Gp100 expression (p<0.05), an increase in tyrosinase activity, and αMSH and TYRP expression within the hair follicle pigmentary unit.
Discussion and Conclusion:
In conclusion, we provide evidence that PS stimulates Gp100 expression and thereby melanogensis in human hair follicles. PS in combination with Gv showed synergic effects in promoting hair pigmentation, primarily through melanosome maturation. Thus, this combinational treatment deserves further pre-clinical and clinical exploration for the reversal of hair greying. In a clinical study with male volunteers, Greyverse™ decreased the overall whiteness of hair and darkened grey hair after three months of treatment leading to an overall reduction of 30% in grey density. It will be important to assess if PS in combination with Gv can further reduce the percentage of canities, similar to our results in ex vivo cultured human HFs.