Protective effect of Ethylhexyl Methoxycinnamate against UV irradation induced hair carbonylation
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Presented by: Seoyeon Kyung
Humans are normally exposed to various external stimuli such as tempereture change, pressure, pH, pollutant and light. The negative effects of solar ultraviolet (UV) radiation, mostly combination of UVB (290-320nm) and UVA (320-400mm) wavelength, have been extensively studied because they are regarded as the main causes of skin aging and hair damage.
When hair undergoes irreversible oxidative damage by UV, hair shows carbonlyated structural transformation resulting in protein loss, ultimately resulting in discoloration and brittleness. Especially, protein and lipid carbonylation is an important indicator of oxidative stress, and it is well known that carbonylation is induced by UVA and UVB.
Ethylhexyl methoxycinnamate, clear and oil soluble organic compound, has been commonly used as chemical sunscreen because of its UVB absorbing capacity. Although it has been widely used as a UV screener for skin, its hair protection effect against UV is not known yet.
We evaluated the effects of ethylhexyl methoxycinnamate on hair protection in human hair. The 7-(diethylamino)-coumarin-3-carbohydrazide (CHH) was used to stain carbonylated proteins of hair after treatment with ethylhexyl methoxycinnamate. Carbonylated phospholipid of hair was stained by E06-monoclonalAb-TopFlour™ antibody. Increased levels of carbonylated protein and phospholipid stained parts of hair by UV were decreased in ethylhexyl methoxycinnamate containg oil compared to control. Furthermore, hair texture softness significantly increased in ethylhexyl methoxycinnamate containing oil treatment group. These results suggest that ethylhexyl methoxycinnamate protect hair texture in human hair through absorbing harmful UV.
When hair undergoes irreversible oxidative damage by UV, hair shows carbonlyated structural transformation resulting in protein loss, ultimately resulting in discoloration and brittleness. Especially, protein and lipid carbonylation is an important indicator of oxidative stress, and it is well known that carbonylation is induced by UVA and UVB.
Ethylhexyl methoxycinnamate, clear and oil soluble organic compound, has been commonly used as chemical sunscreen because of its UVB absorbing capacity. Although it has been widely used as a UV screener for skin, its hair protection effect against UV is not known yet.
We evaluated the effects of ethylhexyl methoxycinnamate on hair protection in human hair. The 7-(diethylamino)-coumarin-3-carbohydrazide (CHH) was used to stain carbonylated proteins of hair after treatment with ethylhexyl methoxycinnamate. Carbonylated phospholipid of hair was stained by E06-monoclonalAb-TopFlour™ antibody. Increased levels of carbonylated protein and phospholipid stained parts of hair by UV were decreased in ethylhexyl methoxycinnamate containg oil compared to control. Furthermore, hair texture softness significantly increased in ethylhexyl methoxycinnamate containing oil treatment group. These results suggest that ethylhexyl methoxycinnamate protect hair texture in human hair through absorbing harmful UV.