Combined proteomics and structural analyses of hair keratins for early detection of hair shafts damage/protection
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Presented by: Martin Baraibar
Introduction
Hair damage induced by external aggressions, such as UV irradiation, fine dust pollution, heat-styling tools, chemical treatments, are tradionnaly assessed by spectroscopic and microscopic imaging or hair physical characteristics tests (shine, strength, suppleness, etc. ). However, these approches are not suitable to evaluate hair damage at daily equivalent stress doses precluding the evaluation of the efficacy of novel products or active compounds in conditions that are closed to consumer’s uses.
Protein carbonylation, a harmful oxidative protein modification, is considered as a major hallmark and a reliable biomarker of oxidative stress. Being the principal components of hair shafts (65% to 95%), proteins are a primary target of oxidative damage upon stress, explaining, at least in part, the molecular basis of the chemical damage to both the hair and scalp. The integration of results from the molecular and structural methods of analysis leads to a 360° vision of early damages on hair provoke by daily life stressor.
Methods
Specific ex-vivo experimental models of daily above mentionned stress, reproducing closed to real life exposures, have been developped using hair tresses and combined to proteomics approaches for the quantification of carbonylated (oxidized) proteins and biophysical methods for the analysis of keratin fibers density and organization. The absolute quantifcation of hair protein oxidation (carbonylation) has been achieved by differential in gel electrophoresis, while the vizualisation and semi-quantification on both cuticle and cortex oxidative damage has been assessed by in situ detection.
Both protection and repair effectiveness of different compounds have been evaluated.
Results
A significant dose-dependent increase in protein oxidation (carbonylation), related to both both keratins, and keratins associated proteins, was observed upon exposures of hair to stress factors, starting from daily stress exposure equivalent doses. However, protein carbonylation was prevented in the presence of anti-oxidant compounds and, interestingly, the application of hair care product treatment on damaged hair tresses resulted in decrease in protein oxidation.
Discussion and Conclusion
Taking together these results lead to a new generations of high-sensitivity and wide-range tools for the assessment of hair protection and repair from daily equivalent stress-induced damages.
Hair damage induced by external aggressions, such as UV irradiation, fine dust pollution, heat-styling tools, chemical treatments, are tradionnaly assessed by spectroscopic and microscopic imaging or hair physical characteristics tests (shine, strength, suppleness, etc. ). However, these approches are not suitable to evaluate hair damage at daily equivalent stress doses precluding the evaluation of the efficacy of novel products or active compounds in conditions that are closed to consumer’s uses.
Protein carbonylation, a harmful oxidative protein modification, is considered as a major hallmark and a reliable biomarker of oxidative stress. Being the principal components of hair shafts (65% to 95%), proteins are a primary target of oxidative damage upon stress, explaining, at least in part, the molecular basis of the chemical damage to both the hair and scalp. The integration of results from the molecular and structural methods of analysis leads to a 360° vision of early damages on hair provoke by daily life stressor.
Methods
Specific ex-vivo experimental models of daily above mentionned stress, reproducing closed to real life exposures, have been developped using hair tresses and combined to proteomics approaches for the quantification of carbonylated (oxidized) proteins and biophysical methods for the analysis of keratin fibers density and organization. The absolute quantifcation of hair protein oxidation (carbonylation) has been achieved by differential in gel electrophoresis, while the vizualisation and semi-quantification on both cuticle and cortex oxidative damage has been assessed by in situ detection.
Both protection and repair effectiveness of different compounds have been evaluated.
Results
A significant dose-dependent increase in protein oxidation (carbonylation), related to both both keratins, and keratins associated proteins, was observed upon exposures of hair to stress factors, starting from daily stress exposure equivalent doses. However, protein carbonylation was prevented in the presence of anti-oxidant compounds and, interestingly, the application of hair care product treatment on damaged hair tresses resulted in decrease in protein oxidation.
Discussion and Conclusion
Taking together these results lead to a new generations of high-sensitivity and wide-range tools for the assessment of hair protection and repair from daily equivalent stress-induced damages.