13:40 - 15:10
Workshop 13, “Development and function of Macrophage and DC”
Room: ANA Crowne Plaza “Ohtori” Room A
Workshop Session
Chair/s:
Frederic Geissmann, Toshiaki Ohteki
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Mapping the human DC lineage through the integration of high-dimensional techniques
Peter See1, Charles-Antoine Dutertre1, 2, Jinmiao Chen1, Patrick Günther3, Naomi McGovern1, Sergio Erdal Irac2, Merry Gunawan4, Marc Beyer5, Kristian Händler3, Kaibo Duan1, Joachim L. Schultze3, 5, Evan W. Newell1, Florent Ginhoux1
1Singapore Immunology Network (SIgN), Singapore, Singapore, 2Program in Emerging Infectious Disease, Duke-NUS Medical School, Singapore, Singapore, 3Genomics and Immunoregulation, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany, 4Institute of Cellular Medicine, Newcastle University,, Newcastle, United Kingdom, 5Platform for Single Cell Genomics and Epigenomics at the German Center for Neurodegenerative Diseases and the University of Bonn, Bonn, Germany

Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.

Reference:
We-WS13-3
Session:
Workshop 13, “Development and function of Macrophage and DC”
Presenter/s:
Peter See
Presentation type:
Oral Presentation
Room:
ANA Crowne Plaza “Ohtori” Room A
Chair/s:
Frederic Geissmann, Toshiaki Ohteki
Date:
Wednesday, 1 November 2017
Time:
14:10 - 14:22
Session times:
13:40 - 15:10
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